Cathepsin A 蛋白, Human (HEK293, His, solution)

Cathepsin A Protein, Human (HEK293, His, solution) is a 58-60 kDa human cathepsin A protein with a His-flag. Cathepsin A is a multicatalytic enzyme with carboxypeptidase activities^[1].

组织蛋白酶 A 作为酶原合成，是一种 54 kDa 的单链前体（信号肽裂解并添加两个 N 连接聚糖后），可自缔合成同型二聚体。前体组织蛋白酶 A 有两个结构域，一个核心结构域（1-182 和 303-452）被帽结构域插入 (183-302) 中断。
组织蛋白酶 A 是一种多催化酶，除了羧肽酶外还具有脱酰胺酶和酯酶
组织蛋白酶 A、溶酶体羧肽酶与 β-半乳糖苷酶是溶酶体多酶复合物的组成部分。组织蛋白酶 A 激活 Neu1 并保护 GAL 和 Neu1 免遭快速蛋白水解降解。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

P10619 (A29-Y480)

5476  [NCBI]

APDQDEIQRLPGLAKQPSFRQYSGYLKGSGSKHLHYWFVESQKDPENSPVVLWLNGGPGCSSLDGLLTEHGPFLVQPDGVTLEYNPYSWNLIANVLYLESPAGVGFSYSDDKFYATNDTEVAQSNFEALQDFFRLFPEYKNNKLFLTGESYAGIYIPTLAVLVMQDPSMNLQGLAVGNGLSSYEQNDNSLVYFAYYHGLLGNRLWSSLQTHCCSQNKCNFYDNKDLECVTNLQEVARIVGNSGLNIYNLYAPCAGGVPSHFRYEKDTVVVQDLGNIFTRLPLKRMWHQALLRSGDKVRMDPPCTNTTAASTYLNNPYVRKALNIPEQLPQWDMCNFLVNLQYRRLYRSMNSQYLKLLSSQKYQILLYNGDVDMACNFMGDEWFVDSLNQKMEVQRRPWLVKYGDSGEQIAGFVKEFSHIAFLTIKGAGHMVPTDKPLAAFTMFSRFLNKQPY

58-60 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filter solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. M Hiraiwa, et al. Cathepsin A/protective protein: an unusual lysosomal multifunctional protein. Cell Mol Life Sci. 1999 Dec;56(11-12):894-907.  [Content Brief]

  [2]. Nilima Kolli, et al. Proteolytic activation of human cathepsin A. J Biol Chem  [Content Brief]