1. 重组蛋白
  2. Cytokines and Growth Factors Receptor Proteins
  3. Chemokine & Receptors G-Protein-Coupled Receptors (GPCRs)
  4. CC Chemokine Receptor Chemokine Receptor
  5. CCR5
  6. CCR5 蛋白, Mouse (P. pastoris, His)

CCR5 蛋白, Mouse (P. pastoris, His)

目录号: HY-P700540
COA 产品使用指南

CCR5 蛋白是炎症 CC 趋化因子(包括 CCL3/MIP-1-alpha、CCL4/MIP-1-beta 和 RANTES)的受体,通过增加细胞内钙离子水平在信号转导中发挥至关重要的作用。它作为趋化受体,有助于粒细胞谱系控制和 T 淋巴细胞迁移至感染部位。与 PRAF2、GRK2、ARRB1、ARRB2 和 CNIH4 的相互作用凸显了 CCR5 监管网络的复杂性。配体与 CCL3/MIP-1alpha 和 CCL4/MIP-1beta 的有效结合需要硫酸化、O-糖基化和唾液酸修饰。Ser-6 上的糖基化对于最佳 CCL4 结合至关重要。在与 S100A4 相互作用中观察到的对 T 淋巴细胞趋化性的刺激作用强调了 CCR5 在免疫反应和细胞信号传导途径中的多方面作用。CCR5 蛋白, Mouse (P. pastoris, His) 是重组的 CCR5 蛋白,由 P. pastoris 酵母表达,带有 N-6*His 标签。CCR5 蛋白, Mouse (P. pastoris, His) 全长 92 个氨基酸,分子量为 12.6 kDa。

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规格 价格 是否有货 数量
20 μg ¥2470 In-stock
50 μg ¥4690 In-stock
100 μg ¥7500 In-stock
> 100 μg   询价  

* Please select Quantity before adding items.

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描述

CCR5 Protein, a receptor for inflammatory CC-chemokines, including CCL3/MIP-1-alpha, CCL4/MIP-1-beta, and RANTES, plays a crucial role in signal transduction by increasing intracellular calcium ion levels. It contributes to granulocytic lineage control and T-lymphocyte migration to infection sites, serving as a chemotactic receptor. Interactions with PRAF2, GRK2, ARRB1, ARRB2, and CNIH4 highlight CCR5's regulatory network complexity. Efficient ligand binding to CCL3/MIP-1alpha and CCL4/MIP-1beta requires sulfation, O-glycosylation, and sialic acid modifications. Glycosylation on Ser-6 is essential for optimal CCL4 binding. The stimulating effect on T-lymphocyte chemotaxis, observed in interaction with S100A4, emphasizes CCR5's multifaceted roles in immune responses and cellular signaling pathways. CCR5 Protein, Mouse (P. pastoris, His) is the recombinant mouse-derived CCR5 protein, expressed by P. pastoris, with N-6*His labeled tag. The total length of CCR5 Protein, Mouse (P. pastoris, His) is 92 a.a., with molecular weight of 12.6 kDa.

研究背景

CCR5 蛋白作为多种炎症 CC 趋化因子(包括 CCL3/MIP-1-α、CCL4/MIP-1-β 和 RANTES)的受体,通过提高细胞内钙离子水平在信号转导中发挥关键作用。该受体可能有助于控制粒细胞谱系增殖或分化,并且是 T 淋巴细胞迁移至感染部位的组成部分,起到趋化受体的作用。与 PRAF2、GRK2、ARRB1、ARRB2 和 CNIH4 的相互作用进一步凸显了 CCR5 监管网络的复杂性。值得注意的是,配体与 CCL3/MIP-1α 和 CCL4/MIP-1β 的有效结合需要硫酸化、O-糖基化和唾液酸修饰。此外,Ser-6 上的糖基化对于最佳 CCL4 结合至关重要。与 S100A4 的相互作用凸显了对 T 淋巴细胞趋化性的刺激作用,强调了 CCR5 在免疫反应和细胞信号传导途径中的多方面作用。

种属

Mouse

表达系统

P. pastoris

标签

N-6*His

蛋白编号

P51682 (Q263-L354)

基因 ID
蛋白结构
N-term
6*His
CCR5 (Q263-L354)
Accession # P51682
C-term
同用名
CCR5; chemokine (C-C motif) receptor 5 (gene/pseudogene); chemokine (C C motif) receptor 5, CMKBR5; C-C chemokine receptor type 5; CC CKR 5; CD195; CKR 5; CKR5; IDDM22; chemr13; HIV-1 fusion coreceptor; chemokine receptor CCR5; C-C motif chemokine receptor 5 A159A; CCR-5; CKR-5; CCCKR5; CMKBR5; CC-CKR-5; FLJ78003;
氨基酸序列

QEFFGLNNCSSSNRLDQAMQATETLGMTHCCLNPVIYAFVGEKFRSYLSVFFRKHMVKRFCKRCSIFQQDNPDRASSVYTRSTGEHEVSTGL

分子量

12.6 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CCR5 Protein, Mouse (P. pastoris, His)
目录号:
HY-P700540
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