1. 重组蛋白
  2. CD Antigens
  3. Macrophage CD Proteins Monocyte CD Proteins Dendritic Cell CD Proteins Epithelial cell CD Proteins
  4. CD14 B7-H3/CD276
  5. CD14 蛋白, Rat (HEK293, Fc)

CD14 蛋白, Rat (HEK293, Fc)

目录号: HY-P74332
产品使用指南

CD14 蛋白充当细菌脂多糖 (LPS) 的共受体,与 LY96 和 TLR4 形成复合物。它将单体 LPS 与 LBP 结合,将其递送至 LY96/TLR4 复合物并启动免疫反应。CD14 蛋白, Rat (HEK293, Fc) 是重组的 CD14 蛋白,由 HEK293 表达,带有 C-hFc 标签。CD14 蛋白, Rat (HEK293, Fc) 全长 341 个氨基酸,分子量约为 ~72 kDa。

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重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

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描述

The CD14 protein acts as a coreceptor for bacterial lipopolysaccharide (LPS), forming a complex with LY96 and TLR4. It binds monomeric LPS to LBP, delivers it to the LY96/TLR4 complex and initiates an immune response. CD14 Protein, Rat (HEK293, Fc) is the recombinant rat-derived CD14 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of CD14 Protein, Rat (HEK293, Fc) is 341 a.a., with molecular weight of ~72 kDa.

研究背景

CD14 蛋白作为细菌脂多糖 (LPS) 的共受体,与 LY96 和 TLR4 形成多蛋白复合物。它与 LBP 合作,结合单体 LPS 并将其递送至 LY96/TLR4 复合物,启动先天免疫反应。CD14 的参与延伸至 TLR2:TLR6 和 TLR2:TLR1 异二聚体,分别响应二酰化和三酰化脂肽。通过与 MyD88、TIRAP 和 TRAF6 相互作用,CD14 激活 NF-kappa-B,导致细胞因子分泌和炎症。此外,它还参与 LDL(-) 诱导的细胞因子释放,并与 LPAR1、MYO18A 和 FSTL1 相互作用,突出了其在免疫和信号通路中的多种作用。

种属

Rat

表达系统

HEK293

标签

C-hFc

蛋白编号

Q63691 (M1-Y341)

基因 ID

60350  [NCBI]

蛋白结构
N-term
CD14 (M1-Y341)
Accession # Q63691
hFc
C-term
同用名
Monocyte Differentiation Antigen CD14; CD14
分子量

Approximately 72 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CD14 Protein, Rat (HEK293, Fc)
目录号:
HY-P74332
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