CD160 蛋白, Human (133a.a, HEK293, His)

CD160 Protein, Human (133a.a, HEK293, His) is a recombinant human CD160 expressed in HEK 293 cells with a His tag at the N-terminus. CD160 Protein binds weakly to MHC I and stimulates NK and CD8⁺ T‐cell activation^[1][2].

CD160 是一种 27 kDa 糖蛋白，是免疫球蛋白蛋白质“超家族”的成员。CD160最初是用单克隆抗体BY55鉴定的。据报道，CD160 由 NK 细胞、NKT 细胞、上皮内 T 细胞、γδ TCR⁺ T 细胞以及记忆表型、激活和效应 CD8⁺ T 细胞表达。CD160 与 MHC I 微弱结合并刺激 NK 和 CD8⁺ T 细胞激活。CD160 还可作为溶细胞或耗竭 CD8⁺ T 细胞^[1][2]的标记。

Human

HEK293

C-6*His

O95971 (I27-S159)

11126  [NCBI]

INITSSASQEGTRLNLICTVWHKKEEAEGFVVFLCKDRSGDCSPETSLKQLRLKRDPGIDGVGEISSQLMFTISQVTPLHSGTYQCCARSQKSGIRLQGHFFSILFTETGNYTVTGLKQRQHLEFSHNEGTLSHHHHHH

20-30 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against 20 mM PB,150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Cai G, et, al. The CD160, BTLA, LIGHT/HVEM pathway: a bidirectional switch regulating T-cell activation. Immunol Rev. 2009 May;229(1):244-58.  [Content Brief]

  [2]. Kaye J. CD160 and BTLA: LIGHTs out for CD4+ T cells. Nat Immunol. 2008 Feb;9(2):122-4.  [Content Brief]