CD2 蛋白, Human (HEK293, His)

CD2 Protein, Human (HEK 293, His) is a transmembrane cell surface glycoprotein found on virtually all T cells, thymocytes, and NK cells. CD2 ligation by CD58 has been shown to mediate T cell adhesion, T cell activation, T cell cytokine production and T cell and NK cells cytotoxic activity.

人类 CD2 分子是一种跨膜细胞表面糖蛋白，几乎存在于所有 T 细胞、胸腺细胞和 NK 细胞上。CD2 促进 T-NK 谱系细胞与抗原呈递细胞 (APC)、基质成分和各种带有普遍表达的 CD2 配体 CD58 的靶细胞的物理相互作用。就 T 细胞而言，CD2 甚至在 T 细胞受体 (TCR) 识别与主要组织相容性复合体 (MHC) 分子 (pMHC) 结合的肽抗原之前就促进了细胞接触的初始阶段。因此，与基于整合素的粘附（例如由 CD11a/CD18 (LFA-1) 介导的粘附）不同，CD2 粘附功能独立于 TCR 触发^[1]。

Human

HEK293

C-6*His

P06729 (K25-D209)

914  [NCBI]

KEITNALETWGALGQDINLDIPSFQMSDDIDDIKWEKTSDKKKIAQFRKEKETFKEKDTYKLFKNGTLKIKHLKTDDQDIYKVSIYDTKGKNVLEKIFDLKIQERVSKPKISWTCINTTLTCEVMNGTDPELNLYQDGKHLKLSQRVITHKWTTSLSAKFKCTAGNKVSKESSVEPVSCPEKGLD

Approximately 34.0 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Wang JH, et, al. Structure of a heterophilic adhesion complex between the human CD2 and CD58 (LFA-3) counterreceptors. Cell. 1999 Jun 11;97(6):791-803.  [Content Brief]