CD3D 蛋白, Mouse (HEK293, Fc)

The CD3D protein is an important component of the TCR-CD3 complex on T lymphocytes and is critical for adaptive immune responses. After APC activates TCR, CD3D, together with CD3E, CD3G and CD3Z, transmits signals through ITAM and activates downstream pathways. CD3D Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived CD3D protein, expressed by HEK293 , with C-hFc labeled tag. The total length of CD3D Protein, Mouse (HEK293, Fc) is 105 a.a., with molecular weight of 48-65 kDa.

CD3D 蛋白是 T 淋巴细胞表面 TCR-CD3 复合物的重要组成部分，在适应性免疫反应中发挥着关键作用。抗原呈递细胞 (APC) 激活 T 细胞受体 (TCR) 后，CD3D 与 CD3E、CD3G 和 CD3Z 一起跨细胞膜传递 TCR 介导的信号。这些 CD3 链的胞质结构域中包含基于免疫受体酪氨酸的激活基序 (ITAM)，在被 LCK 和 FYN 激酶磷酸化后，激活下游信号通路。除了在 T 细胞激活的信号转导中发挥作用外，CD3D 在胸腺细胞分化中也是不可或缺的，有助于细胞内 TCR-CD3 复合物的正确组装和表面表达。TCR-CD3 复合物的功能障碍会导致胸腺细胞分化受损。CD3D 进一步与 CD4 和 CD8 相互作用，在 TCR 与辅助受体 CD4 和 CD8 之间建立功能联系，这对于 CD4 或 CD8 T 细胞的激活和阳性选择至关重要。TCR-CD3 复合物由 CD3D/CD3E 和 CD3G/CD3E 异二聚体组成，形成与 TCRα 和 TCRβ 结合的三聚体。另外，六聚体与CD3Z同型二聚体相互作用以完成TCR-CD3复合物，其中TCRα和TCRβ可以被TCRγ和TCRδ替代。这种复杂的相互作用网络凸显了 CD3D 在协调 T 细胞反应中的多方面作用。

Immobilized Recombinant Human CD3 epsilon Protein at 10 µg/mL (100 µL/well) can bind Biotinylated Mouse CD3D protein. The ED₅₀ for this effect is 4.477 μg/mL.

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  Immobilized Recombinant Human CD3 epsilon Protein at 10 µg/mL (100 µL/well) can bind Biotinylated Mouse CD3D protein. The ED₅₀ for this effect is 4.477 μg/mL.

Mouse

HEK293

C-hFc

P04235 (F22-A105)

12500  [NCBI]

FKIQVTEYEDKVFVTCNTSVMHLDGTVEGWFAKNKTLNLGKGVLDPRGIYLCNGTEQLAKVVSSVQVHYRMCQNCVELDSGTMA

Approximately 48-65 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.