CD43 蛋白, Human (HEK293, His)

CD43 protein is an important leukocyte surface sialic acid protein that plays a key role in T cell regulation. It has a positive impact on T cell function, including activation, proliferation, differentiation, trafficking and migration, especially by helping T cells migrate to lymph nodes through ERM proteins. CD43 Protein, Human (HEK293, His) is the recombinant human-derived CD43 protein, expressed by HEK293 , with C-His labeled tag. The total length of CD43 Protein, Human (HEK293, His) is 234 a.a., with molecular weight of 50-120 kDa.

CD43 蛋白是白细胞的主要细胞表面唾液酸蛋白，在 T 细胞调节中协调多种关键功能。它对 T 细胞活化、增殖、分化、运输和迁移产生积极影响，特别是通过与 ERM 蛋白（EZR、RDX 和 MSN）结合促进 T 细胞运输至淋巴结。CD43 在准备 T 细胞进行细胞因子感应和分化为效应细胞方面发挥作用，同时还负向调节 Th2 细胞分化并引导 T 细胞向 Th1 谱系定型。此外，它在 T 细胞受体 (TCR) 激活过程中诱导 T 细胞表达 IFN-γ、促进 IFNGR 和 IL4R 信号传导以及介导 IFNGR 与 TCR 的聚集方面发挥着关键作用。作为主要的 E-选择素配体，CD43 促进 Th17 细胞在激活的脉管系统上滚动并在炎症期间招募，介导 Th17 细胞与 E-选择素的粘附。此外，CD43 作为 SIGLEC1 的 T 细胞反受体，通过保护细胞免受凋亡信号的影响来促进细胞存活。

Human

HEK293

C-His

P16150 (S20-R253)

6693  [NCBI]

STTAVQTPTSGEPLVSTSEPLSSKMYTTSITSDPKADSTGDQTSALPPSTSINEGSPLWTSIGASTGSPLPEPTTYQEVSIKMSSVPQETPHATSHPAVPITANSLGSHTVTGGTITTNSPETSSRTSGAPVTTAASSLETSRGTSGPPLTMATVSLETSKGTSGPPVTMATDSLETSTGTTGPPVTMTTGSLEPSSGASGPQVSSVKLSTMMSPTTSTNASTVPFRNPDENSR

50-120 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.