CD98 蛋白, Human (HEK293, His)

The CD98 protein acts as a chaperone, promoting the biogenesis and trafficking of functional transporter heterodimers to the plasma membrane. It forms heterodimers with various SLC7 family transporters and affects the substrate specificity of amino acid antiporters. CD98 Protein, Human (HEK293, His) is the recombinant human-derived CD98 protein, expressed by HEK293 , with N-His labeled tag. The total length of CD98 Protein, Human (HEK293, His) is 425 a.a., with molecular weight of 60-80 kDa.

CD98 蛋白作为伴侣，有助于功能性转运蛋白异二聚体的生物发生和运输至质膜。它与各种 SLC7 家族转运蛋白形成异二聚体，包括 SLC7A5、SLC7A6、SLC7A7、SLC7A8、SLC7A10 和 SLC7A11，充当氨基酸反向转运蛋白，其底物特异性由 SLC7A 亚基决定。例如，异二聚体 SLC3A2/SLC7A11 通过介导细胞外阴离子 L-胱氨酸和细胞内 L-谷氨酸的交换而发挥逆向转运蛋白的作用。此外，SLC3A2/SLC7A5 与 LAPTM4B 结合时，会被募集至溶酶体，促进亮氨酸摄取和 mTORC1 激活。CD98 对于各种 SLC7 转运蛋白的质膜定位、稳定性和转运活性至关重要。此外，它在调节整合素相关信号传导方面发挥着关键作用，有助于细胞扩散、迁移和肿瘤进展。在丙型肝炎病毒 (HCV) 感染的情况下，SLC3A2 和 SLC7A5/LAT1 复合物促进病毒进入宿主细胞并增加 L-亮氨酸摄取，从而增强 mTORC1 信号激活并促进 HCV 介导的发病机制。

1.Immobilized CD98, Human His Tag at 0.5 μg/mL (100μl/well) on the plate. Dose response curve for Anti-CD98 Antibody, mFc Tag with the EC₅₀ of 11.1 ng/mL determined by ELISA.
2.Immobilized Human CD98 at 2 μg/mL (100 μL/well) can bind Anti-CD98 Antibody. The ED₅₀ for this effect is 0.03379 nM.

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  Immobilized Human CD98 at 2 μg/mL (100 μL/well) can bind Anti-CD98 Antibody, The ED₅₀ for this effect is 0.03379 nM.

Human

HEK293

N-His

P08195-1 (R206-A630)

6520  [NCBI]

RAPRCRELPAQKWWHTGALYRIGDLQAFQGHGAGNLAGLKGRLDYLSSLKVKGLVLGPIHKNQKDDVAQTDLLQIDPNFGSKEDFDSLLQSAKKKSIRVILDLTPNYRGENSWFSTQVDTVATKVKDALEFWLQAGVDGFQVRDIENLKDASSFLAEWQNITKGFSEDRLLIAGTNSSDLQQILSLLESNKDLLLTSSYLSDSGSTGEHTKSLVTQYLNATGNRWCSWSLSQARLLTSFLPAQLLRLYQLMLFTLPGTPVFSYGDEIGLDAAALPGQPMEAPVMLWDESSFPDIPGAVSANMTVKGQSEDPGSLLSLFRRLSDQRSKERSLLHGDFHAFSAGPGLFSYIRHWDQNERFLVVLNFGDVGLSAGLQASDLPASASLPAKADLLLSTQPGREEGSPLELERLKLEPHEGLLLRFPYAA

60-80 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.