2. 重组蛋白
  3. Enzymes & Regulators
  4. Hydrolases (EC 3)
  5. Cephalosporin C deacetylase 蛋白, Bacillus subtilis

Cephalosporin C deacetylase 蛋白, Bacillus subtilis

目录号: HY-P701944
产品使用指南

头孢菌素 C 脱乙酰酶蛋白是一种酯酶，可选择性地从乙酰化木糖和头孢菌素 C 等底物中去除乙酰基。其催化活性排除了聚合乙酰化木聚糖和酰胺键，突出了其细致入微的靶向脱乙酰功能。Cephalosporin C deacetylase 蛋白, Bacillus subtilis 是重组的 头孢菌素 C 脱乙酰酶蛋白，由 E. coli 表达，不带标签。Cephalosporin C deacetylase 蛋白, Bacillus subtilis 全长 318 个氨基酸。

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描述

Cephalosporin C deacetylase Protein, an esterase, selectively removes acetyl groups from substrates like acetylated xylose and cephalosporin C. Its catalytic activity excludes polymeric acetylated xylan and amide linkages, highlighting its nuanced, targeted deacetylation function. Cephalosporin C deacetylase Protein, Bacillus subtilis is the recombinant Cephalosporin C deacetylase protein, expressed by E. coli , with tag free. The total length of Cephalosporin C deacetylase Protein, Bacillus subtilis is 318 a.a., .
研究背景

头孢菌素 C 脱乙酰酶蛋白起到酯酶的作用，选择性地去除各种 O-乙酰化小底物（包括乙酰化木糖、短低聚木糖和头孢菌素 C）上的乙酰基。值得注意的是，其催化活性不会延伸到聚合乙酰化木聚糖，并且无法裂解酰胺键。这种特异性强调了它在特定底物的靶向脱乙酰化中的作用，强调了其微妙的酶功能。
种属

Others
表达系统

E. coli
标签

Tag Free
蛋白编号

P94388 (M1-G318)
基因 ID

938341
蛋白结构
N-term
CAH (M1-G318)
Accession # P94388
C-term
同用名
cah; Cephalosporin-C deacetylase; Acetylxylan esterase
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Cephalosporin C deacetylase Protein, Bacillus subtilis 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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