Cerberus 1/CER1 蛋白, Human (HEK293, His)

The CER1 protein, a DAN family BMP antagonist, coordinates growth-promoting ureteric bud signaling by inducing BMP inhibition. CER1 also regulates embryogenesis and carcinogenesis by inhibiting NODAL signaling to SMAD2 or SMAD3 through ALK4/ALK7. Cerberus 1/CER1 Protein, Human (HEK293, His) is the recombinant human-derived Cerberus 1/CER1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Cerberus 1/CER1 Protein, Human (HEK293, His) is 250 a.a., with molecular weight of 35-42 kDa.

Cerberus 1, DAN family BMP antagonist (CER1)，是半胱氨酸结超家族蛋白同源物和 DAN 家族 BMP 拮抗剂。CER1 具有九个保守的半胱氨酸和一个半胱氨酸结区域，与 Dan 和 DRM/Gremlin 代表骨形态发生蛋白 (BMP) 拮抗剂，可以直接与 BMP 结合并抑制其活性。人 ES 细胞中 CER1 的上调导致与人 ES 细胞分化相关的 Nodal 信号传导抑制。CER1 (Cerberus 1) 和 GREM3（CKTSF1B3 或 CER2）通过 ACVR1B (ALK4) 或 ACVR1C (ALK7) 抑制 NODAL 信号转导至 SMAD2 或 SMAD3。而 Nodal 和 BMP 信号通路与 WNT 信号通路形成的网络与胚胎发生和癌变过程有关。尽管，CER1 在一定程度上抑制了 Wnt3a 诱导的经典 Top Flash 报告基因，但它并不影响细胞培养中的 Wnt11 信号传导。并且，CER1 通过 Bmp 介导的拮抗作用以及在某种程度上通过参与分支的典型 Wnt 信号来协调促进生长的输尿管芽信号 Gndf 和 Wnt11 的活动，从而微调输尿管树的空间组织。

Human

HEK293

C-6*His

AAI03977.1 (T18-A267)

9350  [NCBI]

TRHQDGRQNQSSLSPVLLPRNQRELPTGNHEEAEEKPDLFVAVPHLVGTSPAGEGQRQREKMLSRFGRFWKKPEREMHPSRDSDSEPFPPGTQSLIQPIDGMKMEKSPLREEAKKFWHHFMFRKTPASQGVILPIKSHEVHWETCRTVPFSQTITHEGCEKLVVQNNLCFGKCGSVHFPGAAQHSHTSCSHCLPAKFTTMHLPLNCTELSSVIKVVMLVEECQCKVKTEHEDGHILHAGSQDSFIPGVSA

35-42 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM NaAc-HAC, pH 4.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.