Cyclophilin F/PPIF 蛋白, Human (HEK293, His)

Cyclophilin F/PPIF Protein, a peptidyl-prolyl cis-trans isomerase (PPIase), catalyzes proline imidic peptide bond isomerization, potentially aiding in protein folding. It crucially regulates the mitochondrial permeability transition pore (mPTP), influencing apoptosis or necrosis. Collaborating with mitochondrial p53/TP53, it activates oxidative stress-induced necrosis and modulates mitochondrial membrane F(1)F(0) ATP synthase activity. Cyclophilin F/PPIF, with BCL2, inhibits cytochrome c-dependent apoptosis independently of the mPTP, highlighting its diverse roles in mitochondrial function and cell survival mechanisms. Cyclophilin F/PPIF Protein, Human (HEK293, His) is the recombinant human-derived Cyclophilin F/PPIF protein, expressed by HEK293, with C-6*His labeled tag. The total length of Cyclophilin F/PPIF Protein, Human (HEK293, His) is 178 a.a., with molecular weight of ~24-34 kDa.

Cyclophilin F/PPIF 蛋白作为肽基-脯氨酰顺反异构酶 (PPIase) 起作用，在催化寡肽中脯氨酸酰亚胺肽键的顺反异构化中发挥着至关重要的作用，从而可能促进蛋白质折叠。除了参与蛋白质折叠之外，亲环蛋白 F/PPIF 还在线粒体通透性转换孔 (mPTP) 的调节中发挥着关键作用，其中它与 mPTP 的关联被认为掩盖了抑制无机磷酸盐 (Pi) 的结合位点，最终促进mPTP开放概率并导致细胞凋亡或坏死；然而，在此过程中是否需要 PPIase 活性仍存在争议。此外，亲环蛋白 F/PPIF 与线粒体 p53/TP53 合作，有助于激活氧化应激诱导的坏死。它进一步参与调节线粒体膜 F(1)F(0) ATP 合酶活性和调节线粒体基质腺嘌呤核苷酸水平。Cyclophilin F/PPIF 具有独立于 mPTP 的抗凋亡活性，与 BCL2 协同作用，可有效抑制细胞色素 c 依赖性细胞凋亡，突出其在线粒体功能和细胞生存机制中的多方面作用。

Human

HEK293

C-6*His

P30405 (C30-S207)

10105

CSKGSGDPSSSSSSGNPLVYLDVDANGKPLGRVVLELKADVVPKTAENFRALCTGEKGFGYKGSTFHRVIPSFMCQAGDFTNHNGTGGKSIYGSRFPDENFTLKHVGPGVLSMANAGPNTNGSQFFICTIKTDWLDGKHVVFGHVKEGMDVVKKIESFGSKSGRTSKKIVITDCGQLS

Approximately 24-34 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.