1. 重组蛋白
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  3. Oxidoreductases (EC 1)
  4. CYP51 蛋白, Mycobacterium tuberculosis (Baculovirus, His-Myc)

CYP51 蛋白, Mycobacterium tuberculosis (Baculovirus, His-Myc)

目录号: HY-P72049
产品使用指南

CYP51 Proteinas 是一种甾醇 14α-去甲基酶,与未知的生理底物作用并接受来自铁硫铁氧还蛋白 Fdx 的电子。在体外,它催化羊毛甾醇、24,25-二氢羊毛甾醇和奥坦醇的 C14-去甲基化,在产生 8,14-二烯时表现出立体选择性。CYP51 蛋白, Mycobacterium tuberculosis (Baculovirus, His-Myc) 是重组的 CYP51 蛋白,由 Sf9 insect cells 表达,带有 N-10*His, C-Myc 标签。CYP51 蛋白, Mycobacterium tuberculosis (Baculovirus, His-Myc) 全长 451 个氨基酸,分子量约为 ~54.8 kDa。

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描述

CYP51 Proteinas is a sterol 14α-demethylase that acts with an unknown physiological substrate and accepts electrons from the iron-sulfur ferredoxin Fdx. In vitro, it catalyzes the C14-demethylation of lanosterol, 24,25-dihydrolanosterol, and obtanyol, showing stereoselectivity in producing 8,14-diene. CYP51 Protein, Mycobacterium tuberculosis (Baculovirus, His-Myc) is the recombinant CYP51 protein, expressed by Sf9 insect cells , with N-10*His, C-Myc labeled tag. The total length of CYP51 Protein, Mycobacterium tuberculosis (Baculovirus, His-Myc) is 451 a.a., with molecular weight of ~54.8 kDa.

研究背景

CYP51 蛋白被鉴定为甾醇 14α-去甲基酶,与未知的生理底物一起作用,并接收来自邻近基因编码的铁硫铁氧还蛋白 Fdx 的电子。体外实验证明其能够催化羊毛甾醇、24,25-二氢羊毛甾醇和钝叶醇的 C14-去甲基化,在产生 8,14-二烯方面表现出立体选择性。这种酶活性强调了其在甾醇生物合成中的关键作用,特别是在生成 8,14-二烯等必需中间体方面。

种属

Others

表达系统

Sf9 insect cells

标签

N-10*His;C-Myc

蛋白编号

P9WPP8 (M1-V451)

基因 ID

/

蛋白结构
N-term
10*His
CYP51 (M1-V451)
Accession # P9WPP8
Myc
C-term
同用名
cyp51; MT0788Lanosterol 14-alpha demethylase; EC 1.14.14.154; CYPLI; Cytochrome P450 51; Cytochrome P450-14DM; Cytochrome P450-LIA1; Sterol 14-alpha demethylase
氨基酸序列

MSAVALPRVSGGHDEHGHLEEFRTDPIGLMQRVRDECGDVGTFQLAGKQVVLLSGSHANEFFFRAGDDDLDQAKAYPFMTPIFGEGVVFDASPERRKEMLHNAALRGEQMKGHAATIEDQVRRMIADWGEAGEIDLLDFFAELTIYTSSACLIGKKFRDQLDGRFAKLYHELERGTDPLAYVDPYLPIESFRRRDEARNGLVALVADIMNGRIANPPTDKSDRDMLDVLIAVKAETGTPRFSADEITGMFISMMFAGHHTSSGTASWTLIELMRHRDAYAAVIDELDELYGDGRSVSFHALRQIPQLENVLKETLRLHPPLIILMRVAKGEFEVQGHRIHEGDLVAASPAISNRIPEDFPDPHDFVPARYEQPRQEDLLNRWTWIPFGAGRHRCVGAAFAIMQIKAIFSVLLREYEFEMAQPPESYRNDHSKMVVQLAQPACVRYRRRTGV

分子量

Approximately 54.8 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

CYP51 Protein, Mycobacterium tuberculosis (Baculovirus, His-Myc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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