DAPK1 蛋白, Human (sf9, His-GST)

The DAPK1 protein is a calcium/calmodulin-dependent kinase involved in multiple cellular pathways, regulating cell survival, apoptosis, and autophagy. The DAPK1 protein also acts as a signal amplifier for NMDA receptors, causing brain damage in stroke. DAPK1 Protein, Human (sf9, His-GST) is the recombinant human-derived DAPK1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of DAPK1 Protein, Human (sf9, His-GST) is 363 a.a., with molecular weight of ~64 kDa.

DAPK1 蛋白是一种钙/钙调蛋白依赖性丝氨酸/苏氨酸激酶，复杂地参与多种细胞信号传导途径，调节细胞存活、凋亡和自噬。其多功能作用包括协调 I 型凋亡和 II 型自噬细胞死亡信号，前者依赖于 caspase，后者的特点是自噬囊泡的积累且不依赖于 caspase。DAPK1 参与多种底物的磷酸化事件：它抑制 PIN1 的催化活性、核定位和细胞功能，通过磷酸化 TPM1 增强内皮细胞中的应力纤维形成，并显着降低 STX1A 与 STXBP1 的结合。此外，它磷酸化 PRKD1 以调节氧化应激下的 JNK 信号传导，并通过磷酸化 BECN1 来调节自噬，破坏其与 BCL2 和 BCL2L1 的相互作用。DAPK1 还会磷酸化 TSC2，破坏 TSC1-TSC2 复合物并刺激生长因子依赖性途径中的 mTORC1 活性。在突触外 NMDA 受体的背景下，DAPK1 充当信号放大器，通过 NMDA 受体通道诱导有害的 Ca(2+) 流入，介导中风中的脑损伤。此外，DAPK1 与 DAPK3 协同作用，在干扰素 γ 激活后磷酸化 RPL13A，从而导致转录选择性翻译抑制。值得注意的是，DAPK1 的亚型 2 缺乏诱导细胞凋亡的能力，但保留了诱导膜起泡的能力。

The specific activity was determined to be 20 nmol/min/mg using synthetic R11-S6-Peptide as substrate.

Human

Sf9 insect cells

N-His;N-GST

P53355-1 (M1-L363)

1612  [NCBI]

Approximately 64 kDa

Greater than 80% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 10% gly

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.