DNASE1L3 蛋白, Human (GST)

DNASE1L3 proteolytically cleaves single- and double-stranded DNA and generates fragments with 3'-OH termini. It can cleave chromatin, nucleosomes, and liposome-coated DNA. DNASE1L3 Protein, Human (GST) is the recombinant human-derived DNASE1L3 protein, expressed by E. coli , with N-GST labeled tag. The total length of DNASE1L3 Protein, Human (GST) is 285 a.a., with molecular weight of ~60.4 kDa.

DNASE1L3 蛋白具有 DNA 水解活性，能够熟练地切割单链和双链 DNA，从而产生带有 3'-OH 末端的 DNA 片段。该蛋白质可以将染色质切割成核小体单位，并对核小体和脂质体包被的 DNA 表现出活性。它在细胞凋亡和坏死过程中的核小体间 DNA 断裂中发挥着至关重要的作用，有助于肌源性和神经元分化等过程，以及 BCR 介导的自身反应性 B 细胞的克隆缺失。DNASE1L3 对凋亡细胞来源的膜包被微粒中的染色质具有活性，从而抑制抗 DNA 自身免疫。此外，DNASE1L3 与 DNASE1 合作，对于降解中性粒细胞胞外陷阱 (NET) 至关重要，NET 主要由中性粒细胞在炎症过程中释放的 DNA 纤维组成。DNASE1 和 DNASE1L3 对血管内 NET 的降解对于防止血栓形成至关重要，血栓可能会阻塞血管并导致炎症后的器官损伤。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-GST

Q13609 (M21-S305)

1776  [NCBI]

MRICSFNVRSFGESKQEDKNAMDVIVKVIKRCDIILVMEIKDSNNRICPILMEKLNRNSRRGITYNYVISSRLGRNTYKEQYAFLYKEKLVSVKRSYHYHDYQDGDADVFSREPFVVWFQSPHTAVKDFVIIPLHTTPETSVKEIDELVEVYTDVKHRWKAENFIFMGDFNAGCSYVPKKAWKNIRLRTDPRFVWLIGDQEDTTVKKSTNCAYDRIVLRGQEIVSSVVPKSNSVFDFQKAYKLTEEEALDVSDHFPVEFKLQSSRAFTNSKKSVTLRKKTKSKRS

Approximately 60.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.