1. 重组蛋白
  2. Cytokines and Growth Factors
  3. TNF Superfamily
  4. TNF Receptor Superfamily
  5. EDA2R
  6. EDA2R/XEDAR 蛋白, Rat (HEK293, His)

EDA2R/XEDAR 蛋白, Rat (HEK293, His)

目录号: HY-P76313
COA 产品使用指南

EDA2R/XEDAR 蛋白缺乏对特征注释传播至关重要的保守残基,并表现出独特的特征,引发了对其结构和功能方面的疑问。这种独特性表明分子相互作用和信号通路的潜在变化。EDA2R/XEDAR 蛋白, Rat (HEK293, His) 是重组的 EDA2R/XEDAR 蛋白,由 HEK293 表达,带有 C-His 标签。EDA2R/XEDAR 蛋白, Rat (HEK293, His) 全长 136 个氨基酸,分子量约为 ~24 KDa。

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The EDA2R/XEDAR proteins lack conserved residues critical for feature annotation propagation and exhibit unique characteristics that raise questions about their structural and functional aspects. This uniqueness suggests potential changes in molecular interactions and signaling pathways. EDA2R/XEDAR Protein, Rat (HEK293, His) is the recombinant rat-derived EDA2R/XEDAR protein, expressed by HEK293 , with C-His labeled tag. The total length of EDA2R/XEDAR Protein, Rat (HEK293, His) is 136 a.a., with molecular weight of ~24 KDa.

研究背景

EDA2R,也称为 XEDAR,具有独特的特征,因为它缺乏特征注释传播所需的保守残基。这种独特的特征引发了有关 EDA2R 结构和功能方面的有趣问题,暗示其分子相互作用和信号传导途径的潜在变化。这些保守残基的缺失凸显了深入研究的必要性,以阐明与 EDA2R 相关的具体作用和调节机制,揭示其对细胞过程和生物功能的贡献。

生物活性

Immobilized Recombinant Rat EDA2R at 0.1 μg/mL (100 μL/well) can bind Recombinant Human EDA-A2. The ED50 for this effect is 14.16 ng/mL.

种属

Rat

表达系统

HEK293

标签

C-His

蛋白编号

D3ZAX4 (M2-E137)

基因 ID

296872  [NCBI]

蛋白结构
N-term
EDA2R (M2-E137)
Accession # D3ZAX4
His
C-term
同用名
Tumor necrosis factor receptor superfamily member 27; EDA-A2 receptor; EDA2R; TNFRSF27; XEDAR
氨基酸序列

MDCQENEYRDQWGRCVTCQQCGPGQELSKDCGYGEGGDAYCIACPPRKYKSTWGHHRCQTCITCAVINRVQKANCTNTSNAICGDCLPRFYRKTRIGGLQDQECIPCTKQTPSSEVQCTFQLSLVKVDTHTLPPKE

分子量

Approximately 24 kDa due to the glycosylation.

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

EDA2R/XEDAR Protein, Rat (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
EDA2R/XEDAR Protein, Rat (HEK293, His)
目录号:
HY-P76313
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