1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. ENTPD3 蛋白, Human (sf9, His)

ENTPD3 蛋白, Human (sf9, His)

目录号: HY-P76904
COA 产品使用指南

ENTPD3 蛋白表现出独特的酶偏好,对 ATP 水解具有三重水解特异性,对 ADP 水解具有三重特异性。这显示出对 ATP 底物水解的明显亲和力,表明在涉及 ATP 分子分解的细胞过程中发挥着关键作用。ENTPD3 蛋白, Human (sf9, His) 是重组的 ENTPD3 蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。ENTPD3 蛋白, Human (sf9, His) 全长 442 个氨基酸,分子量约为 ~50 kDa。

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规格 价格 是否有货 数量
10 μg ¥1600 In-stock
50 μg ¥4500 In-stock
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The ENTPD3 protein exhibits unique enzymatic preferences, with triple hydrolysis specificity for ATP hydrolysis and triple specificity for ADP hydrolysis. This shows a clear affinity for ATP substrate hydrolysis, suggesting a critical role in cellular processes involving the breakdown of ATP molecules. ENTPD3 Protein, Human (sf9, His) is the recombinant human-derived ENTPD3 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of ENTPD3 Protein, Human (sf9, His) is 442 a.a., with molecular weight of ~50 kDa.

研究背景

ENTPD3 蛋白表现出对 ATP(三磷酸腺苷)水解的明显偏好,而不是对 ADP(二磷酸腺苷)水解的偏好。这种酶的特异性意味着对裂解 ATP 中的高能磷酸键具有特殊的亲和力,突出了其在水解这种必需的三磷酸核苷酸中的作用。对 ATP 水解的偏好表明 ENTPD3 可能在调节细胞能量动态方面发挥重要作用,可能参与需要 ATP 作为能量来源的细胞过程。进一步的研究可能会深入了解与 ENTPD3 酶活性相关的特定细胞途径和功能及其对细胞生物能学的影响。

生物活性

Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-triphosphate (ATP) and the specific activity is >70,000 pmol/min/µg.

种属

Human

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

O75355 (Q44-P485)

基因 ID

956  [NCBI]

蛋白结构
N-term
ENTPD3 (Q44-P485)
Accession # O75355
His
C-term
同用名
Ectonucleoside triphosphate diphosphohydrolase 3; NTPDase 3; Ecto-apyrase 3; CD39L3
分子量

Approximately 50 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 10% gly

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

ENTPD3 Protein, Human (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
ENTPD3 Protein, Human (sf9, His)
目录号:
HY-P76904
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