Ephrin-A5/EFNA5 蛋白, Human (HEK293, Fc)

Ephrin-A5/EFNA5 Protein, a cell surface GPI-bound ligand, crucially interacts with Eph receptors, inducing bidirectional signaling and regulating adhesion, organization, and development in neurons, vasculature, and epithelium. It forms complexes with EPHA3, EPHA8, and ADAM10, impacting internalization and function, while also mediating communication in pancreatic islet cells and influencing brain development. Ephrin-A5/EFNA5 Protein, Human (HEK293, Fc) is the recombinant human-derived Ephrin-A5/EFNA5 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Ephrin-A5/EFNA5 Protein, Human (HEK293, Fc) is 183 a.a., with molecular weight of 55-60 kDa.

Ephrin-A5/EFNA5 蛋白是一种细胞表面 GPI 结合配体，通过与 Eph 受体（受体酪氨酸激酶家族）相互作用，在神经元、血管和上皮发育中发挥至关重要的作用。这种相互作用导致相邻细胞之间依赖于接触的双向信号传导。Ephrin-A5/EFNA5 当与其同源受体结合时，会在类似小窝的膜微域内诱导区室化信号传导，并且该信号传导需要 Fyn 酪氨酸激酶的活性。它激活 EPHA3 受体来调节细胞间粘附和细胞骨架组织，还可能参与维持晶状体透明度和刺激轴突束颤。此外，Ephrin-A5/EFNA5 介导胰岛细胞之间的通讯，以调节葡萄糖刺激的胰岛素分泌，并通过与 EPHA7 的相互作用调节细胞间粘附和排斥来调节大脑发育。此外，Ephrin-A5/EFNA5 与 EPHB2 结合并与 EPHA8 相互作用，激活后者。它还与 EPHA2、EPHA3 和 ADAM10 形成复合物，调节 Ephrin-A5/EFNA5 的脱落和内化，从而影响其功能。

Human

HEK293

C-hFc

P52803 (Q21-N203)

1946  [NCBI]

QDPGSKAVADRYAVYWNSSNPRFQRGDYHIDVCINDYLDVFCPHYEDSVPEDKTERYVLYMVNFDGYSACDHTSKGFKRWECNRPHSPNGPLKFSEKFQLFTPFSLGFEFRPGREYFYISSAIPDNGRRSCLKLKVFVRPTNSCMKTIGVHDRVFDVNDKVENSLEPADDTVHESAEPSRGEN

55-60 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.