eptA 蛋白, E.coli strain K12 (Cell-Free, His)

The eptA protein plays a crucial role in bacterial resistance to polymyxins by catalyzing the addition of a phosphoethanolamine moiety to lipid A. This modification is essential for conferring resistance to the antimicrobial effects of polymyxins. eptA Protein, E.coli strain K12 (Cell-Free, His) is the recombinant E. coli-derived eptA protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of eptA Protein, E.coli strain K12 (Cell-Free, His) is 547 a.a., with molecular weight of 64.5 kDa.

EptA 是一种催化磷酸乙醇胺部分添加到脂质 A 上的酶，脂质 A 是细菌细胞膜中脂多糖 (LPS) 的成分。这种修饰对于赋予多粘菌素抗性至关重要，多粘菌素是一种针对细菌外膜的抗生素。脂质 A 中添加的磷酸乙醇胺改变了其结构，有助于细菌针对多粘菌素诱导的膜破坏的防御机制。EptA 已被鉴定为复合物的一部分，其中一个已知成分是 ZipA-EptA 融合体，以及一种未鉴定的 24 kDa 蛋白质。这表明 EptA 的功能与复合物中的其他蛋白质错综复杂地联系在一起，可能形成一个涉及细菌膜完整性和抗菌剂耐药性的协调系统。

E.coli

E. coli Cell-free

N-10*His

P30845 (M1-E547)

75169632

MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDSLHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDRSMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGANILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVRIGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTASCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACDRVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNRYPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSLVYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQTQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE

64.5 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.