1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. ERK3 蛋白, Human (His)

ERK3 蛋白, Human (His)

目录号: HY-P701680
产品使用指南

ERK3 蛋白是一种非典型 MAPK,磷酸化 MAP2 和 MAPKAPK5。ERK3/MAPK6 与 MAPKAPK5 相互作用,发生 Ser-189 磷酸化,促进 MAPKAPK5 激活。激活的 MAPKAPK5 会相互磷酸化 ERK3/MAPK6。该复合物的精确功能意义,特别是在细胞周期进入方面,仍然难以捉摸。ERK3 蛋白, Human (His) 是重组的 ERK3 蛋白,由 E. coli 表达,带有 N-6*His 标签。ERK3 蛋白, Human (His) 全长 319 个氨基酸。

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描述

ERK3 Protein, an atypical MAPK, phosphorylates MAP2 and MAPKAPK5. Interacting with MAPKAPK5, ERK3/MAPK6 undergoes Ser-189 phosphorylation, facilitating MAPKAPK5 activation. Activated MAPKAPK5 reciprocally phosphorylates ERK3/MAPK6. The complex's precise functional implications, especially in cell cycle entry, remain elusive. ERK3 Protein, Human (His) is the recombinant human-derived ERK3 protein, expressed by E. coli , with N-6*His labeled tag. The total length of ERK3 Protein, Human (His) is 319 a.a., .

研究背景

ERK3 是一种非典型 MAPK 蛋白,具有磷酸化微管相关蛋白 2 (MAP2) 和 MAPKAPK5 的能力。与 MAPKAPK5 形成的复合物的复杂性仍在研究中,但在这种相互作用中发生了一系列磷酸化事件。与非典型 MAPKAPK5 结合后,ERK3/MAPK6 在 Ser-189 处发生磷酸化,随后促进 MAPKAPK5 的磷酸化和激活。反过来,激活的 MAPKAPK5 会相互磷酸化 ERK3/MAPK6。这种复杂的相互作用的精确功能含义仍然难以捉摸,尽管它可能在促进进入细胞周期方面发挥作用。

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

Q16659 (M9-I327)

基因 ID

5597

蛋白结构
N-term
6*His
ERK3 (M9-I327)
Accession # Q16659
C-term
同用名
MAPK6; Mitogen-activated protein kinase 6; MAP kinase 6; MAPK 6; Extracellular signal-regulated kinase 3; ERK-3; MAP kinase isoform p97; p97-MAPK
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

ERK3 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ERK3 Protein, Human (His)
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