1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. EXTL2 蛋白, Mouse (HEK293, His)

EXTL2 蛋白, Mouse (HEK293, His)

目录号: HY-P70908
产品使用指南

EXTL2 蛋白是硫酸乙酰肝素生物合成中的关键糖基转移酶,负责监督 β-1-4-连接的葡萄糖醛酸 (GlcA) 和 α-1-4-连接的 N-乙酰葡糖胺 (GlcNAc) 单元的转化 添加交替的硫酸盐链到新生的乙酰肝素。这种酶的功能对于硫酸乙酰肝素的复杂和精确构建至关重要,硫酸乙酰肝素是各种生物过程的关键组成部分。EXTL2 蛋白, Mouse (HEK293, His) 是重组的 EXTL2 蛋白,由 HEK293 表达,带有 N-6*His 标签。EXTL2 蛋白, Mouse (HEK293, His) 全长 288 个氨基酸,分子量约为 ~35.0 kDa。

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描述

The EXTL2 protein is a key glycosyltransferase in heparan sulfate biosynthesis, responsible for overseeing the conversion of β-1-4-linked glucuronic acid (GlcA) and α-1-4-linked N-acetylglucosamine (GlcNAc) units Alternating sulfate chains are added to the nascent heparan. The function of this enzyme is critical for the complex and precise construction of heparan sulfate, a key component of various biological processes. EXTL2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived EXTL2 protein, expressed by HEK293 , with N-6*His labeled tag. The total length of EXTL2 Protein, Mouse (HEK293, His) is 288 a.a., with molecular weight of ~35.0 kDa.

研究背景

EXTL2(外骨蛋白样 2)是一种糖基转移酶,对于硫酸乙酰肝素(一种复杂且必需的糖胺聚糖)的生物合成至关重要。其酶活性涉及将 β-1-4 连接的葡萄糖醛酸 (GlcA) 和 α-1-4 连接的 N-乙酰葡糖胺 (GlcNAc) 单元交替添加到新生的硫酸乙酰肝素链上。这种酶促过程是构建硫酸乙酰肝素复杂多糖结构不可或缺的一部分,而硫酸乙酰肝素反过来又在各种细胞功能中发挥关键作用,包括细胞信号传导、粘附和组织发育。

种属

Mouse

表达系统

HEK293

标签

N-6*His

蛋白编号

Q9ES89 (N43-M330)

基因 ID

58193  [NCBI]

蛋白结构
N-term
6*His
EXTL2 (N43-M330)
Accession # Q9ES89
C-term
同用名
Exostosin-like 2; Extl2; Alpha-1; 4-N-acetylhexosaminyltransferase EXTL2; Alpha-GalNAcT EXTL2; EXT-related protein 2; Glucuronyl-galactosyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase
氨基酸序列

NIKEDKMLTLRREIKSPSKSALDSFTLIMQTYNRTDLLLRLLNHYQAVPSLHKVIVVWNNVGEKGPEELWNSLGPHPIPVIFKPQTANKMRNRLQVFPEVETNAVLMVDDDTLISAQDLVFAFSIWQQFPDQIIGFVPRKHVSTSSGIYSYGGFELQTPGPGNGDQYSMVLIGASFFNSKYLELFQKQPAAVHALIDETQNCDDIAMNFLVTRHTGKPSGIFVKPINMVNLEKETNGYSGMWHRAEHFLQRSYCINKLVNIYDGMPLKYSNIMISQFGFPYANHKSKM

分子量

Approximately 35.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

EXTL2 Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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EXTL2 Protein, Mouse (HEK293, His)
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