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  3. FBXO48 蛋白, Human (His)

FBXO48 蛋白, Human (His)

目录号: HY-P71558
产品使用指南

FBXO48 蛋白是 SCF 泛素连接酶复合物的一部分,参与 SCF 依赖性蛋白酶体泛素依赖性蛋白分解代谢过程。FBXO48 蛋白 通过靶向活性磷酸化的 Ampkα (pAmpkα) 进行多泛素化和蛋白酶体降解。FBXO48 蛋白, Human (His) 是重组的 FBXO48 蛋白,由 E. coli 表达,带有 N-His, C-Myc 标签。

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描述

The FBXO48 Protein is part of the SCF ubiquitin ligase complex and participates in the catabolism of SCF-dependent proteasome ubiquitin-dependent proteins. The FBXO48 Protein undergoes polyubiquitination and proteasome degradation by targeting activity-phosphorylated Ampk伪 (PAMPK-伪). FBXO48 Protein, Human (His) is the recombinant human-derived FBXO48 protein, expressed by E. coli , with N-His, C-Myc labeled tag.

研究背景

F-box only protein 48 是 SCF 泛素连接酶复合物的一部分,参与 SCF 依赖性蛋白酶体泛素依赖性蛋白分解代谢过程。FBXO48 通过靶向活性磷酸化的 Ampkα (pAmpkα) 进行多泛素化和蛋白酶体降解。FBXO48基因位于帕金森病 3 型 (PARK3) 致病基因位点 2p13.3,是帕金森病 15 型 (PARK15) 致病基因 F-box only protein 7 基因 (FBXO7) 的同源基因之一,也被称为帕金森锥体病 (PPD)。

种属

Human

表达系统

E. coli

标签

N-His;C-Myc

蛋白编号

Q5FWF7 (M1-R155)

基因 ID

554251  [NCBI]

蛋白结构
N-term
10*His
FBXO48 (M1-R155)
Accession # Q5FWF7
Myc
C-term
同用名
FBXO48; FBX48F-box only protein 48
氨基酸序列

MHKNSKRNNNLRVSHTEANSVDAEKEKNESQNNFFELLPAEITFKIFSQLDIRSLCRASLTCRSWNDTIRNSDSLWKPHCMTVRAVCRREIDDDLESGYSWRVILLRNYQKSKVKHEWLSGRYSNICSPISLPEKIMYPMDADTWGEILEAELER

分子量

Approximately 25.7 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

FBXO48 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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FBXO48 Protein, Human (His)
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