Frizzled-10/CD350 蛋白, Human (HEK293, His)

The Frizzled-10/CD350 gene is a member of the Frizzled gene family, encoding a 7-transmembrane domain protein that acts as a receptor for the Wingless-type MMTV integration site family signaling protein. Most Frizzled receptors, including Frizzled-10/CD350, are involved in cell signaling, specifically the β-catenin classical pathway. Frizzled-10/CD350 Protein, Human (HEK293, His) is the recombinant human-derived Frizzled-10/CD350 protein, expressed by HEK293 , with C-His labeled tag. The total length of Frizzled-10/CD350 Protein, Human (HEK293, His) is 141 a.a., with molecular weight of 20-25 kDa.

Frizzled-10/CD350 基因属于卷曲基因家族，编码 7 次跨膜结构域蛋白，作为信号蛋白 Wingless 型 MMTV 整合位点家族的受体。大多数卷曲受体通常与 β-连环蛋白经典信号传导途径相关，在细胞信号传导中发挥着至关重要的作用。值得注意的是，通过阵列分析，这种无内含子基因的表达在两个原发性结肠癌实例中表现出显着的上调。这表明 Frizzled-10/CD350 可能参与结肠癌，从而促使进一步探索其对信号通路和癌症进展的具体贡献。

Measured by its ability to bind biotinylated recombinant mouse Wnt-3a in a functional ELISA. The ED₅₀ for this effect is 4.336 nM.

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  Measured by its ability to bind biotinylated recombinant mouse Wnt-3a in a functional ELISA. The ED₅₀ for this effect is 4.336nM.

Human

HEK293

C-6*His

NP_009128.1 (I21-G161)

11211  [NCBI]

ISSMDMERPGDGKCQPIEIPMCKDIGYNMTRMPNLMGHENQREAAIQLHEFAPLVEYGCHGHLRFFLCSLYAPMCTEQVSTPIPACRVMCEQARLKCSPIMEQFNFKWPDSLDCRKLPNKNDPNYLCMEAPNNGSDEPTRG

Approximately 20-25 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.