Fusion glycoprotein F0 蛋白, Human metapneumovirus (Cell-Free, His, SUMO)

Fusion glycoprotein F0 is cleaved to generate mature F1 and F2 fusion glycoproteins. It transitions through different conformational states to promote the fusion of virus and cell membranes. Fusion glycoprotein F0 Protein, Human metapneumovirus (Cell-Free, His, SUMO) is the recombinant Virus-derived Fusion glycoprotein F0 protein, expressed by E. coli Cell-free , with N-6*His, N-SUMO labeled tag. The total length of Fusion glycoprotein F0 Protein, Human metapneumovirus (Cell-Free, His, SUMO) is 521 a.a., with molecular weight of 72.4 kDa.

融合糖蛋白 F0 是一种无活性前体，经过裂解生成成熟的 F1 和 F2 融合糖蛋白，代表 I 类病毒融合蛋白。根据目前的模型，该蛋白质至少表现出三种构象状态：融合前天然状态、发夹前中间状态和融合后发夹状态。在病毒和浆细胞膜融合事件中，卷曲螺旋区域采用发夹三聚体结构，将融合肽对齐到胞外域的 C 末端区域附近。这种结构的形成驱动病毒和细胞膜的并置和随后的融合，促进核衣壳递送到细胞质中。值得注意的是，这种融合过程与 pH 值无关，并且发生在质膜或内体膜上。F1-F2 三聚体（F 蛋白）还通过与宿主硫酸乙酰肝素结合，在宿主细胞附着中发挥关键作用，突出了其在介导病毒进入和感染中的多方面作用。

Virus

E. coli Cell-free

N-6*His;N-SUMO

Q6WB98 (L19-S539)

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LKESYLEESCSTITEGYLSVLRTGWYTNVFTLEVGDVENLTCSDGPSLIKTELDLTKSALRELKTVSADQLAREEQIENPRQSRFVLGAIALGVATAAAVTAGVAIAKTIRLESEVTAIKNALKTTNEAVSTLGNGVRVLATAVRELKDFVSKNLTRAINKNKCDIDDLKMAVSFSQFNRRFLNVVRQFSDNAGITPAISLDLMTDAELARAVSNMPTSAGQIKLMLENRAMVRRKGFGILIGVYGSSVIYMVQLPIFGVIDTPCWIVKAAPSCSGKKGNYACLLREDQGWYCQNAGSTVYYPNEKDCETRGDHVFCDTAAGINVAEQSKECNINISTTNYPCKVSTGRHPISMVALSPLGALVACYKGVSCSIGSNRVGIIKQLNKGCSYITNQDADTVTIDNTVYQLSKVEGEQHVIKGRPVSSSFDPIKFPEDQFNVALDQVFENIENSQALVDQSNRILSSAEKGNTGFIIVIILIAVLGSSMILVSIFIIIKKTKKPTGAPPELSGVTNNGFIPHS

72.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.