1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. FUT10 蛋白, Human (HEK293, His)

FUT10 蛋白, Human (HEK293, His)

目录号: HY-P76941
产品使用指南

FUT10 蛋白在糖基化中发挥着关键作用,主要是对双触角 N-聚糖中最内部的 GlcNAc 残基进行岩藻糖基化。该活性产生核心 α(1->3)-岩藻糖表位,作为错误折叠糖蛋白靶向降解的识别信号。FUT10 蛋白, Human (HEK293, His) 是重组的 FUT10 蛋白,由 HEK293 表达,带有 N-His 标签。FUT10 蛋白, Human (HEK293, His) 全长 448 个氨基酸,分子量为 55-65 kDa。

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描述

The FUT10 protein plays a key role in glycosylation, primarily fucosylating the innermost GlcNAc residues in biantennary N-glycans. This activity generates a core α(1->3)-fucose epitope that serves as a recognition signal for targeted degradation of misfolded glycoproteins. FUT10 Protein, Human (HEK293, His) is the recombinant human-derived FUT10 protein, expressed by HEK293 , with N-His labeled tag. The total length of FUT10 Protein, Human (HEK293, His) is 448 a.a., with molecular weight of 55-65 kDa.

研究背景

FUT10 蛋白通过主要对双触角 N-聚糖受体中最里面的 N-乙酰氨基葡萄糖 (GlcNAc) 残基进行岩藻糖基化,在糖基化过程中占据中心位置。推测这种酶活性会在双触角 N-聚糖的壳二糖单元内产生核心 α(1->3)-岩藻糖表位,作为异常折叠糖蛋白靶向降解的识别信号。此外,FUT10 在携带 Lewis X 的双触角 N-聚糖的生物合成中发挥着至关重要的作用,有助于调节大脑发育过程中神经元干细胞的自我更新。该酶催化岩藻糖基部分从 GDP-β-L-岩藻糖转移到双触角 N-聚糖受体中最里面的 GlcNAc 残基,同时显着避免 2 型乳糖胺单元内的 GlcNAc 岩藻糖基化。

种属

Human

表达系统

HEK293

标签

N-His

蛋白编号

Q6P4F1 (L32-D479)

基因 ID
蛋白结构
N-term
His
FUT10 (L32-D479)
Accession # Q6P4F1
C-term
同用名
Alpha-(1,3)-fucosyltransferase 10; Fucosyltransferase X; Fuc-TX; Fucosyltransferase 10
分子量

55-65 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

FUT10 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
FUT10 Protein, Human (HEK293, His)
目录号:
HY-P76941
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