2. 重组蛋白
  3. Viral Proteins
  4. Influenza Viruses Proteins
  5. Influenza Hemagglutinin
  6. HA/Hemagglutinin 蛋白, Influenza B (ACN29380, sf9)

HA/Hemagglutinin 蛋白, Influenza B (ACN29380, sf9)

目录号: HY-P73947
COA 产品使用指南

血凝素 (HA) 蛋白通过与含唾液酸的受体结合，将病毒颗粒附着到宿主细胞上，并通过网格蛋白依赖性内吞作用诱导病毒颗粒内化。HA 还促进一些病毒颗粒的另一种独立于网格蛋白和小窝蛋白的途径。HA/Hemagglutinin 蛋白, Influenza B (ACN29380, sf9) 是重组的 HA/血凝素蛋白，由 Sf9 insect cells 表达，不带标签。HA/Hemagglutinin 蛋白, Influenza B (ACN29380, sf9) 全长 585 个氨基酸，分子量约为 ~61.7 kDa。

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描述

The hemagglutinin (HA) protein attaches viral particles to host cells by binding to sialic acid-containing receptors and induces viral particle internalization through clathrin-dependent endocytosis. HA also promotes an alternative clathrin- and caveolin-independent pathway for some virions. HA/Hemagglutinin Protein, Influenza B (ACN29380, sf9) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by Sf9 insect cells , with tag free. The total length of HA/Hemagglutinin Protein, Influenza B (ACN29380, sf9) is 585 a.a., with molecular weight of ~61.7 kDa.
研究背景

血凝素 (HA) 蛋白通过与细胞表面含唾液酸的受体结合，在病毒颗粒附着至宿主细胞的过程中发挥着至关重要的作用。这种相互作用不仅通过网格蛋白依赖性内吞作用诱导病毒粒子内化，而且还促进了大约三分之一的病毒颗粒的另一种网格蛋白和小窝蛋白独立途径。HA是I类病毒融合蛋白，负责通过介导病毒颗粒膜与内体膜的融合来穿透细胞质。内体中的低pH环境会触发HA2的不可逆构象变化，导致融合疏水肽的释放。几个三聚体的协同作用对于形成有能力的融合孔是必要的，这凸显了HA在宿主范围限制和毒力测定中的复杂作用。
种属

Virus
表达系统

Sf9 insect cells
标签

Tag Free
蛋白编号

ACN29380 (M1-L585)
基因 ID

/
蛋白结构
N-term
HA (M1-L585)
Accession # ACN29380
C-term
同用名
HA; Hemagglutinin; HA/Hemagglutinin Protein, Influenza B (B/Brisbane/60/2008, sf9)
分子量

Approximately 61.7 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
性状

溶液
组分

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, 10% Glycerol, pH 7.5.
内毒素含量

<1 EU/μg, determined by LAL method.
复溶方法

N/A.
保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.
文件资料

COA (147 KB)

产品使用指南 (1213 KB)

HA/Hemagglutinin Protein, Influenza B (ACN29380, sf9) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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