HA/Hemagglutinin 蛋白, H1N1 (ACP41105, sf9)

The hemagglutinin (HA) protein attaches viral particles to host cells by binding to sialic acid-containing receptors and induces viral particle internalization through clathrin-dependent endocytosis. HA also promotes an alternative clathrin- and caveolin-independent pathway for some virions. HA/Hemagglutinin Protein, H1N1 (ACP41105, sf9) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by Sf9 insect cells , with tag free. The total length of HA/Hemagglutinin Protein, H1N1 (ACP41105, sf9) is 566 a.a., with molecular weight of ~61.5 KDa.

血凝素 (HA) 蛋白通过与细胞表面含唾液酸的受体结合，在病毒颗粒附着至宿主细胞的过程中发挥着至关重要的作用。这种相互作用不仅通过网格蛋白依赖性内吞作用诱导病毒粒子内化，而且还促进了大约三分之一的病毒颗粒的另一种网格蛋白和小窝蛋白独立途径。HA是I类病毒融合蛋白，负责通过介导病毒颗粒膜与内体膜的融合来穿透细胞质。内体中的低pH环境会触发HA2的不可逆构象变化，导致融合疏水肽的释放。几个三聚体的协同作用对于形成有能力的融合孔是必要的，这凸显了HA在宿主范围限制和毒力测定中的复杂作用。

Virus

Sf9 insect cells

Tag Free

ACP41105 (M1-I566)

/

Approximately 61.5 kDa.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, 10% Glycerol, pH7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.