2. 重组蛋白
  3. Enzymes & Regulators Ubiquitin Related Proteins
  4. Transferases (EC 2) Ubiquitin Enzymes
  5. E3 Ligases
  6. HLTF 蛋白, Human

HLTF 蛋白, Human

目录号: HY-P701528
产品使用指南

HLTF 蛋白具有解旋酶和 E3 泛素连接酶活性，并具有内在的 ATP 依赖性核小体重塑能力。它对于 SERPINE1、HIV-1 和 SV40 等特定启动子的转录调控至关重要。HLTF 蛋白, Human 是重组的 HLTF 蛋白，由 E. coli 表达，不带标签。HLTF 蛋白, Human 全长 117 个氨基酸。

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规格 是否有货
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100 μg   询价  

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描述

HLTF protein has helicase and E3 ubiquitin ligase activities and has intrinsic ATP-dependent nucleosome remodeling ability. It is critical for the transcriptional regulation of specific promoters such as SERPINE1, HIV-1 and SV40. HLTF Protein, Human is the recombinant human-derived HLTF protein, expressed by E. coli , with tag free. The total length of HLTF Protein, Human is 117 a.a., .
研究背景

HLTF 蛋白具有解旋酶和 E3 泛素连接酶活性。凭借内在的 ATP 依赖性核小体重塑能力，它可能对于 SERPINE1、HIV-1 和 SV40 等特定靶启动子的转录调节至关重要。此外，HLTF 在受损 DNA 的无差错复制后修复 (PRR) 中发挥着至关重要的作用，通过充当泛素连接酶催化染色质结合 PCNA 的“Lys-63”连接多泛素化，从而有助于基因组稳定性。这些功能凸显了其在维持基因组过程和调控机制完整性方面的重要性。
种属

Human
表达系统

E. coli
标签

Tag Free
蛋白编号

Q14527 (V58-A174)
基因 ID

6596
蛋白结构
N-term
HLTF (V58-A174)
Accession # Q14527
C-term
同用名
HLTF; Helicase-like transcription factor; DNA-binding protein/plasminogen activator inhibitor 1 regulator; HIP116; RING finger protein 80; RING-type E3 ubiquitin transferase HLTF; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3; Sucrose nonfermenting protein 2-like 3
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

HLTF Protein, Human 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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