HN1 蛋白, Mouse (His)

HN1 protein negatively regulates AKT-mediated GSK3B signaling and induces the phosphorylation and degradation of CTNNB1 at “Ser-33”. This effect inhibits the “Ser-9” phosphorylation of GSK3B, inhibits the activity of APC:CTNNB1:GSK3B complex and blocks its interaction with CDH1. HN1 Protein, Mouse (His) is the recombinant mouse-derived HN1 protein, expressed by E. coli , with N-His labeled tag. The total length of HN1 Protein, Mouse (His) is 154 a.a., with molecular weight of ~18.3 kDa.

HN1 蛋白成为细胞过程中的关键调节因子，对 AKT 介导的 GSK3B 信号传导发挥负调节作用。它通过诱导 CTNNB1 在“Ser-33”位点的磷酸化和随后的降解来发挥作用。该作用是通过抑制 GSK3B 的抑制性“Ser-9”磷酸化来实现的，从而抑制 APC:CTNNB1:GSK3B 复合物的活性并阻止其与粘附连接中的 CDH1/E-钙粘蛋白相互作用。除了在细胞粘附中的作用之外，HN1 还参与细胞周期动力学的调节，其对这些基本细胞过程的影响就证明了这一点。此外，HN1 在 AR 信号通路中表现出抑制作用，有助于受体的蛋白酶体降解。值得注意的是，HN1 参与与 APC:CTNNB1:GSK3B 复合物的相互作用，特别是与“Ser-9”处磷酸化的 GSK3B 的非活性形式的相互作用，强调了它参与复杂的细胞信号传导网络。

Mouse

E. coli

N-His

P97825 (M1-G154)

15374  [NCBI]

MTTTTTFKGVDPNSRNSSRVLRPPGGGSNFSLGFDEPAEQPVRKNKMASNIFGTPEENPPSWAKSAGSKSSGGREDSESPGTQRSNSSEASSGDFLDLKGEGDMHENVDTDFQANLAQMEEKPVPAAPVPSPVAPAPVPSRRNPPGGKSSLVLG

Approximately 24 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.