IL-1 beta 蛋白, Rat (His)

IL-1 beta protein is a potent proinflammatory cytokine and endogenous pyrogen that induces multiple inflammatory responses, including prostaglandin synthesis, neutrophil, T-cell and B-cell activation, and fibroblast proliferation. It promotes Th17 differentiation, synergizes with IL12 to promote IFNG synthesis, and induces VEGF production through TNF and IL6 to promote angiogenesis. IL-1 beta Protein, Rat (His) is the recombinant rat-derived IL-1 beta protein, expressed by E. coli , with N-6*His labeled tag. The total length of IL-1 beta Protein, Rat (His) is 152 a.a., with molecular weight of ~19 kDa.

IL-1 β 蛋白是一种有效的促炎细胞因子，最初被发现是一种主要的内源性热原。它诱导各种炎症反应，包括前列腺素合成、中性粒细胞激活、T细胞激活和细胞因子产生、B细胞激活和抗体产生、以及成纤维细胞增殖和胶原蛋白产生。它还促进 T 细胞的 Th17 分化，并与 IL12 协同诱导 Th1 细胞合成 IFNG。IL-1 β 蛋白通过与 TNF 和 IL6 协同诱导 VEGF 产生，在血管生成中发挥作用。此外，它还参与细胞焦亡下游炎症的转导，其成熟形式通过gasdermin-D (GSDMD) 孔特异性释放到细胞外空间。IL-1 β 蛋白以单体形式存在并与 MEFV 相互作用。

Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells.The ED₅₀ for this effect is 3.285 ng/mL, corresponding to a specific activity is 3.044×10⁵ units/mg.

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  Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells.The ED₅₀ for this effect is 3.285 ng/mL, corresponding to a specific activity is 3.044×10⁵ units/mg.

Rat

E. coli

N-6*His

Q5BKB0 (V117-S268)

24494  [NCBI]

VPIRQLHCRLRDEQQKCLVLSDPCELKALHLNGQNISQQVVFSMSFVQGETSNDKIPVALGLKGKNLYLSCVMKDGTPTLQLESVDPKQYPKKKMEKRFVFNKIEVKTKVEFESAQFPNWYISTSQAEHRPVFLGNSNGRDIVDFTMEPVSS

Approximately 19 kDa

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  Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Stapp JM, et al. Recombinant rat IL-1beta and IL-6 synergistically enhance C3 mRNA levels and complementcomponent C3 secretion by H-35 rat hepatoma cells. Cytokine. 2005 Apr 21;30(2):78-85.  [Content Brief]