IL-1RA/IL-1F3 蛋白, Porcine

The IL-1RA/IL-1F3 protein, a robust anti-inflammatory antagonist in the interleukin-1 family, specifically targets proinflammatory cytokines IL1B and IL1A. Countering IL1's inflammatory effects, this protein crucially protects against immune dysregulation and prevents uncontrolled systemic inflammation triggered by various innate stimulatory agents, including pathogens. Serving as a regulatory shield against IL1-mediated responses, IL-1RA/IL-1F3 significantly contributes to maintaining immune balance and curtailing excessive inflammatory reactions. IL-1RA/IL-1F3 Protein, Porcine is the recombinant Porcine-derived IL-1RA/IL-1F3 protein, expressed by E. coli, with tag free. The total length of IL-1RA/IL-1F3 Protein, Porcine is 152 a.a., with molecular weight of ~17.55 kDa.

IL-1RA/IL-1F3 蛋白是 IL-1 家族中的有效抗炎拮抗剂，专门针对促炎细胞因子，如 IL-1β/IL1B 和 IL-1α/IL1A。由于能够对抗 IL1 的炎症效应，该蛋白在防止免疫失调和预防由多种先天刺激剂（包括病原体）引起的不受控制的全身炎症方面发挥着至关重要的作用。通过充当针对 IL1 介导的反应的调节屏障，IL-1RA/IL-1F3 对维持免疫平衡和减少过度炎症反应有显着贡献。

Measured by its ability to inhibit IL-1 alpha -dependent proliferation in CTLL-2. The ED₅₀ for this effect is 0.03295 μg/mL in the presence of 50 pg/mL of recombinant porcine IL-1 alpha, corresponding to a specific activity is 4.18×10^4 units/mg.

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  Measured by its ability to inhibit IL-1 alpha -dependent proliferation in CTLL-2. The ED₅₀ for this effect is 0.03295 μg/mL in the presence of 50 pg/mL of recombinant porcine IL-1 alpha, corresponding to a specific activity is 4.18×10⁴ units/mg.

Porcine

E. coli

Tag Free

Q29056 (H26-Q177)

397499  [NCBI]

HPLGKRPCRMQAFRIWDVNQKTFYLRNNQLVAGYLQGPNTKLEEKIDVVPVEPHFVFLGIHGGKLCLSCVKSGDEMKLQLDAVNITDLRKNSEQDKRFTFIRSDSGPTTSFESAACPGWFLCTALEADQPVGLTNTPKAAVKVTKFYFQQDQ

Approximately 17.55 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.