IL-1RA/IL-1RN 蛋白, Rat (HEK293, Fc)

IL-1RA/IL-1RN mutant proteins are potent anti-inflammatory antagonists of the interleukin 1 family, specifically targeting the proinflammatory cytokines IL1B and IL1A. This mutant variant is designed for enhanced suppressive capacity, tightly protecting the host from immune dysregulation and preventing IL1 from triggering uncontrolled systemic inflammation in response to innate stimulators. IL-1RA/IL-1RN Protein, Rat (HEK293, Fc) is the recombinant rat-derived IL-1RA/IL-1RN protein, expressed by HEK293 , with N-hFc labeled tag. The total length of IL-1RA/IL-1RN Protein, Rat (HEK293, Fc) is 152 a.a., with molecular weight of ~41.81 kDa.

IL-1RA/IL-1RN 突变蛋白在 IL-1 家族中充当有效的抗炎拮抗剂，专门针对促炎细胞因子，如 IL-1β/IL1B 和 IL-1α/IL1A。这种突变体经过精心设计，可增强其抑制能力，在保护宿主免受免疫失调和防止IL1响应各种先天刺激剂（包括病原体）而引发不受控制的全身炎症方面发挥着关键作用。通过提供针对 IL1 介导的炎症反应的优化防御，突变蛋白对维持免疫平衡和防止过度炎症做出了重大贡献。

Measured by its ability to inhibit IL-1 alpha -dependent proliferation in CTLL‑2 mouse cytotoxic T cells. The ED₅₀ for this effect is 99.07 ng/mL in the presence of 50 pg/mL of IL-1 alpha, corresponding to a specific activity is 1.009×10^4 units/mg.

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  Measured by its ability to inhibit IL-1 alpha -dependent proliferation in CTLL‑2 mouse cytotoxic T cells. The ED₅₀ for this effect is 99.07 ng/mL in the presence of 50 pg/mL of IL-1 alpha, corresponding to a specific activity is 1.009×10₄ units/mg.

Rat

HEK293

N-hFc

P25086 (H27-Q178)

60582  [NCBI]

HPAGKRPCKMQAFRIWDTNQKTFYLRNNQLIAGYLQGPNTKLEEKIDMVPIDFRNVFLGIHGGKLCLSCVKSGDDTKLQLEEVNITDLNKNKEEDKRFTFIRSETGPTTSFESLACPGWFLCTTLEADHPVSLTNTPKEPCTVTKFYFQEDQ

Approximately 41.81 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.