IL-36 alpha/IL-1F6 蛋白, Mouse (153a.a)

IL-36 alpha (IL-1F6), a subform of IL-36 family, belongs to IL-1 superfamily. IL-36 alpha mediates inflammatory response. IL-36 alpha binds to IL-36R and activates NF-κB and MAPK signaling pathways, but the activation requires N-terminal cleavage by neutrophil granule-derived proteases^[1]. IL-36 alpha also binds to IL-1Rrp2 and recruit IL-1RAcP. IL-36 alpha activats the MAPK, Erk1/2 and JNK through IL-36R/IL-1RAcP^[2]. IL-36 alpha/IL-1F6 Protein, Mouse (153a.a) is a recombinant mouse IL-36 alpha (R8-H160) without any tag, which is produced in E. coli.

IL-36α是IL-36家族的一个亚型，属于IL-1超家族。IL-36 α 在单核细胞、T/B 淋巴细胞、脾脏、骨髓扁桃体、淋巴结和皮肤中表达^[2]。IL-36α的氨基酸序列在不同物种中有所不同。人 IL-36 α 与小鼠的氨基酸序列同一性<55%。
IL-36α与IL-36R结合并激活NF-κB和MAPK信号通路，从而介导炎症反应。但激活需要中性粒细胞颗粒衍生的蛋白酶（例如组织蛋白酶 G、弹性蛋白酶和蛋白酶-3^[1]）进行 N 末端裂解。IL-36 α 还可以结合 IL-1Rrp2 并募集 IL-1RAcP。IL-36 α 通过 IL-36R/IL-1RAcP^[2] 激活 MAPK、Erk1/2 和 JNK。感染后IL-36α显着升高。IL-36 α 在调节巨噬细胞的抗菌功能中发挥着关键作用，这是预防小鼠败血症所必需的^[3]。IL-36 α是真菌性角膜炎免疫反应中的促炎因子，可促进角膜炎症^[4
IL-36 α是与一些感染性和免疫性相关的促炎细胞因子疾病。IL-36 α 通过激活 NF-κB 和 MAPK 信号通路介导炎症反应^[1]。

IL-36 alpha (mouse) (10 µg, intratracheal administration) induces neutrophil influx in the lungs of wild-type C57BL/6 mice and IL-1αβ^−/− mice^[5].
IL-36 alpha (mouse) (1 µg, i.p.) improves survival in sepsis mice^[3].
IL-36 alpha (mouse) (1 µg/5 µL, subconjunctival injection) aggravates the inflammatory response in mice^[4].

Mouse

E. coli

Tag Free

Q9JLA2 (R8-H160)

54448  [NCBI]

RAASPSLRHVQDLSSRVWILQNNILTAVPRKEQTVPVTITLLPCQYLDTLETNRGDPTYMGVQRPMSCLFCTKDGEQPVLQLGEGNIMEMYNKKEPVKASLFYHKKSGTTSTFESAAFPGWFIAVCSKGSCPLILTQELGEIFITDFEMIVVH

Approximately 16 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Zhou L,et al. Interleukin-36: Structure, Signaling and Function. Adv Exp Med Biol. 2021;21:191-210.  [Content Brief]

  [2]. Gresnigt MS, et al. Biology of IL-36 cytokines and their role in disease. Semin Immunol. 2013 Dec 15;25(6):458-65.  [Content Brief]

  [3]. Tao X, Song Z, Wang C, Luo H, Luo Q, Lin X, Zhang L, Yin Y, Cao J. Interleukin 36α Attenuates Sepsis by Enhancing Antibacterial Functions of Macrophages. J Infect Dis. 2017 Jan 15;215(2):321-332.  [Content Brief]

  [4]. Niu Y, et al. IL-36α Exerts Proinflammatory Effects in Aspergillus fumigatus Keratitis of Mice Through the Pathway of IL-36α/IL-36R/NF-κB. Invest Ophthalmol Vis Sci. 2021 Apr 1;62(4):16.  [Content Brief]

  [5]. Ramadas RA, et al. IL-36α exerts pro-inflammatory effects in the lungs of mice. PLoS One. 2012;7(9):e45784.  [Content Brief]