IRAK4 蛋白, Human (sf9, His)

IRAK4 Protein, a serine/threonine-protein kinase, is pivotal in initiating innate immune responses via Toll-like receptor (TLR) and IL-1R signaling pathways. Upon TLR activation, it rapidly forms the Myddosome with IRAK2. IRAK4 phosphorylates IRAK1, promoting its autophosphorylation, and phosphorylates Pellino proteins to facilitate IRAK1 polyubiquitination. This leads to NF-kappa-B activation and regulates immune responses during microbial infections. Additionally, it phosphorylates TIRAP, influencing its ubiquitination and degradation. IRAK4 Protein, Human (sf9, His) is the recombinant human-derived IRAK4 protein, expressed by Sf9 insect cells , with N-10*His labeled tag. The total length of IRAK4 Protein, Human (sf9, His) is 460 a.a., with molecular weight of ~48 kDa.

IRAK4 蛋白作为关键的丝氨酸/苏氨酸蛋白激酶发挥作用，在启动针对外来病原体的先天免疫反应中发挥着至关重要的作用。它积极参与 Toll 样受体 (TLR) 和 IL-1R 信号通路，并且在 TLR 激活后，它被 MYD88 快速招募，与 IRAK2 结合形成 Myddosome。最初，IRAK1 被磷酸化，刺激其激酶活性并诱导强烈的自磷酸化。此外，IRAK4 磷酸化 E3 泛素连接酶 Pellino 蛋白（PELI1、PELI2 和 PELI3），促进 Pellino 介导的 IRAK1 多泛素化。因此，多泛素化的IRAK1与IKBKG/NEMO的泛素结合域结合，组装IRAK1-MAP3K7/TAK1-TRAF6复合物和NEMO-IKKA-IKKB复合物。这反过来又激活 IKK（CHUK/IKKA 和 IKBKB/IKKB），导致核转位和 NF-kappa-B 激活。或者，TIRAP 被 IRAK4 磷酸化，促进其泛素化和随后的降解。此外，IRAK4 磷酸化 NCF1，NCF1 在 LPS 刺激后调节 NADPH 氧化酶的激活，这表明微生物感染期间存在类似的机制。

The specific activity was determined to be >169 nmol/min/mg using MBP as substrate.

Human

Sf9 insect cells

N-10*His

Q9NWZ3-1 (M1-S460)

51135  [NCBI]

MNKPITPSTYVRCLNVGLIRKLSDFIDPQEGWKKLAVAIKKPSGDDRYNQFHIRRFEALLQTGKSPTSELLFDWGTTNCTVGDLVDLLIQNEFFAPASLLLPDAVPKTANTLPSKEAITVQQKQMPFCDKDRTLMTPVQNLEQSYMPPDSSSPENKSLEVSDTRFHSFSFYELKNVTNNFDERPISVGGNKMGEGGFGVVYKGYVNNTTVAVKKLAAMVDITTEELKQQFDQEIKVMAKCQHENLVELLGFSSDGDDLCLVYVYMPNGSLLDRLSCLDGTPPLSWHMRCKIAQGAANGINFLHENHHIHRDIKSANILLDEAFTAKISDFGLARASEKFAQTVMTSRIVGTTAYMAPEALRGEITPKSDIYSFGVVLLEIITGLPAVDEHREPQLLLDIKEEIEDEEKTIEDYIDKKMNDADSTSVEAMYSVASQCLHEKKNKRPDIKKVQQLLQEMTAS

Approximately 48 kDa

Greater than 90% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 0.5 mM PMSF, pH 7.4

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.