JNK1 蛋白, Human (sf9, GST)

JNK1 is a multifunctional serine/threonine protein kinase that complexly regulates multiple cellular processes, including cell proliferation, apoptosis, and differentiation. JNK1 is activated by MAP2K4/MKK4 and MAP2K7/MKK7, phosphorylates AP-1 components, and affects transcriptional activity. JNK1 Protein, Human (sf9, GST) is the recombinant human-derived JNK1 protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of JNK1 Protein, Human (sf9, GST) is 427 a.a., with molecular weight of ~65 kDa.

JNK1 是一种丝氨酸/苏氨酸蛋白激酶，复杂地调节多种细胞过程，包括细胞增殖、分化、迁移、转化和程序性细胞死亡。应激激活蛋白激酶/c-Jun N 末端激酶 (SAP/JNK) 信号通路在促炎细胞因子或身体应激等细胞外因素的刺激下被激活，涉及双特异性激酶对 JNK1 的磷酸化和激活MAP2K4/MKK4 和 MAP2K7/MKK7。在随后的级联反应中，JNK1 磷酸化关键转录因子，特别是 AP-1 的成分，例如 JUN、JDP2 和 ATF2，从而调节 AP-1 转录活性。JNK1 还通过磷酸化复制许可因子 CDT1，破坏其与组蛋白 H4 乙酰化酶 HBO1 的相互作用，对复制起始进行调节控制。该事件阻碍了复制起点所需的乙酰化。此外，JNK1 通过磷酸化关键调节因子（包括 p53/TP53 和 Yes 相关蛋白 YAP1），在细胞凋亡诱导中发挥关键作用。它参与多种细胞途径，包括对 T 细胞分化、红系细胞存活、自噬激活和神经元微管动力学调节的贡献。JNK1 广泛的底物库包括热休克因子蛋白 4 (HSF4)、脱乙酰酶 SIRT1、ELK1、E3 连接酶 ITCH、生物钟调节器 CLOCK-BMAL1 和热休克转录因子 HSF1 等。值得注意的是，JNK1 同工型表现出不同的结合模式，β-1 优先与 c-Jun 结合，而 alpha-1、alpha-2 和 beta-2 显示出与 c-Jun 和 ATF2 相似的低水平结合，尽管磷酸化不同异构体的效率保持一致。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-GST

P45983 (M1-R427)

5599  [NCBI]

Approximately 65 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, pH 8.0, 25% glycerol.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.