2. 重组蛋白
  3. Enzymes & Regulators
  4. Transferases (EC 2)
  5. Kanamycin kinase type II/NEO 蛋白, Klebsiella pneumoniae (Tag Free)

Kanamycin kinase type II/NEO 蛋白, Klebsiella pneumoniae (Tag Free)

目录号: HY-P70228A
产品使用指南

卡那霉素激酶 II 型/NEO 蛋白赋予对氨基糖苷类抗生素（包括卡那霉素、新霉素、巴龙霉素、核糖霉素、布替罗辛和庆大霉素 B）的抗性。它可以抵消它们的抑制作用，展示了该蛋白在减轻各种抗生素的影响和使生物体发挥作用方面的多功能性在他们的存在下茁壮成长。Kanamycin kinase type II/NEO 蛋白, Klebsiella pneumoniae (Tag Free) 是重组的 卡那霉素激酶 II 型/NEO 蛋白，由 E. coli 表达，不带标签。Kanamycin kinase type II/NEO 蛋白, Klebsiella pneumoniae (Tag Free) 全长 264 个氨基酸，分子量约为 approximately 29.68 kDa。

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规格 价格 是否有货
5 μg ￥700 询问价格 & 货期
10 μg ￥1200 询问价格 & 货期
50 μg ￥3600 询问价格 & 货期
100 μg ￥6100 询问价格 & 货期

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描述

The Kanamycin kinase type II/NEO protein imparts resistance to aminoglycoside antibiotics, including kanamycin, neomycin, paromomycin, ribostamycin, butirosin, and gentamicin B. It counteracts their inhibitory effects, showcasing the protein's versatility in mitigating the impact of various antibiotics and enabling the organism to thrive in their presence. Kanamycin kinase type II/NEO protein, Klebsiella pneumoniae (Tag Free) is the recombinant Kanamycin kinase type II/NEO protein, expressed by E. coli , with tag free. The total length of Kanamycin kinase type II/NEO protein, Klebsiella pneumoniae (Tag Free) is 264 a.a., with molecular weight of approximately 29.68 kDa.
研究背景

卡那霉素激酶 II/NEO 蛋白赋予对一系列氨基糖苷类抗生素的耐药性，包括卡那霉素、新霉素、巴龙霉素、核糖霉素、布替罗辛和庆大霉素 B。其作用包括减轻这些抗生素的影响，使生物体能够承受其抑制作用。这种耐药机制凸显了卡那霉素激酶 II 型/NEO 蛋白的多功能性，因为它可以对抗氨基糖苷类抗生素的多种成员，从而有助于生物体在这些抗菌剂存在下茁壮成长的能力。
种属

Others
表达系统

E. coli
标签

Tag Free
蛋白编号

P00552 (M1-F264)
基因 ID

59690385
蛋白结构
N-term
NEO (M1-F264)
Accession # P00552
C-term
同用名
rKlAminoglycoside 3'-phosphotransferase; Aminoglycoside 3'-phosphotransferase; APH(3')-II; APH(3')II; Kanamycin kinase type II; Neomycin-kanamycin phosphotransferase type II; neo
氨基酸序列

MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF

分子量

approximately 29.68 kDa
纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Kanamycin kinase type II/NEO protein, Klebsiella pneumoniae (Tag Free) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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