KCNJ10 蛋白, Rat (Cell-Free, His)

The KCNJ10 protein is an inwardly rectifying potassium channel that plays a critical buffering role in potassium in brain glial cells. It facilitates the influx of potassium and is regulated at extracellular levels. KCNJ10 Protein, Rat (Cell-Free, His) is the recombinant rat-derived KCNJ10 protein, expressed by E. coli Cell-free , with N-6*His labeled tag. The total length of KCNJ10 Protein, Rat (Cell-Free, His) is 379 a.a., with molecular weight of 46.6 kDa.

KCNJ10 蛋白与大脑神经胶质细胞的钾缓冲作用有关。作为一种内向整流钾通道，KCNJ10 表现出明显的偏好，允许钾流入细胞而不是流出。其电压依赖性通过细胞外钾浓度进行微调，导致随着外部钾水平升高而转向更正的电压。内向整流机制主要归因于内部镁对外向电流的阻断，并且可以受到细胞外钡和铯的阻碍。在肾脏中，KCNJ10 与 KCNJ16 协同介导远端肾小管基底外侧 K(+) 循环，这是 Na(+) 重吸收的关键过程。KCNJ10 与 Kir5.1/KCNJ16 形成异二聚体，确保 KCNJ16 定位到肾细胞基底外侧膜所需的相互作用。此外，KCNJ10 与 MAGI1 相互作用，既独立又可能与 KCNJ16 形成异二聚体，可能促进肾细胞基底外侧膜 KCNJ10/KCNJ16 钾通道的表达。此外，KCNJ10 与 PATJ 相互作用，暗示其更广泛地参与细胞相互作用和膜定位。

Rat

E. coli Cell-free

N-6*His

P49655 (M1-V379)

29718

MTSVAKVYYSQTTQTESRPLVAPGIRRRRVLTKDGRSNVRMEHIADKRFLYLKDLWTTFIDMQWRYKLLLFSATFAGTWFLFGVVWYLVAVAHGDLLELGPPANHTPCVVQVHTLTGAFLFSLESQTTIGYGFRYISEECPLAIVLLIAQLVLTTILEIFITGTFLAKIARPKKRAETIRFSQHAVVAYHNGKLCLMIRVANMRKSLLIGCQVTGKLLQTHQTKEGENIRLNQVNVTFQVDTASDSPFLILPLTFYHVVDETSPLKDLPLRSGEGDFELVLILSGTVESTSATCQVRTSYLPEEILWGYEFTPAISLSASGKYVADFSLFDQVVKVASPGGLRDSTVRYGDPEKLKLEESLREQAEKEGSALSVRISNV

46.6 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.