1. 重组蛋白
  2. Ubiquitin Related Proteins
  3. KLHL42 蛋白, Human (Sf9, His, Strep)

KLHL42 蛋白, Human (Sf9, His, Strep)

目录号: HY-P701588
产品使用指南

KLHL42 作为 BCR E3 泛素连接酶复合物中的底物特异性连接子,在有丝分裂进展和胞质分裂中发挥关键作用。BCR(KLHL42) 复合物主动泛素化并降解 KATNA1,有助于有丝分裂期间微管动力学的调节。KLHL42 蛋白, Human (Sf9, His, Strep) 是重组的 KLHL42 蛋白,由 Sf9 insect cells 表达,带有 N-Strep, N-8*His 标签。KLHL42 蛋白, Human (Sf9, His, Strep) 全长 504 个氨基酸。

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描述

KLHL42 serves as a substrate-specific linker in the BCR E3 ubiquitin ligase complex and plays a critical role in mitotic progression and cytokinesis. The BCR(KLHL42) complex actively ubiquitinates and degrades KATNA1, contributing to the regulation of microtubule dynamics during mitosis. KLHL42 Protein, Human (Sf9, His, Strep) is the recombinant human-derived KLHL42 protein, expressed by Sf9 insect cells , with N-Strep, N-8*His labeled tag. The total length of KLHL42 Protein, Human (Sf9, His, Strep) is 504 a.a., .

研究背景

KLHL42 作为 BCR (BTB-CUL3-RBX1) E3 泛素-蛋白质连接酶复合物内的底物特异性接头,在有丝分裂进展和胞质分裂中发挥关键作用。BCR(KLHL42) E3 泛素连接酶复合物通过主动介导 KATNA1 的泛素化和随后的降解来协调这些重要的细胞过程,从而有助于调节整个有丝分裂过程中的微管动力学。KLHL42 参与蛋白质修饰,特别是蛋白质泛素化,凸显了其在微调细胞事件方面的重要性,这些事件对于正确的有丝分裂进程和胞质分裂至关重要。BCR(KLHL42) 复合物内错综复杂的相互作用强调了其在维持与细胞分裂相关的动态过程所需的微妙平衡方面的重要性。

种属

Human

表达系统

Sf9 insect cells

标签

N-Strep;N-8*His

蛋白编号

Q9P2K6 (S2-T505)

基因 ID

57542

蛋白结构
N-term
8*His-Strep
KLHL42 (S2-T505)
Accession # Q9P2K6
C-term
同用名
KLHL42; Kelch-like protein 42; Cullin-3-binding protein 9; Ctb9; Kelch domain-containing protein 5
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

KLHL42 Protein, Human (Sf9, His, Strep) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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KLHL42 Protein, Human (Sf9, His, Strep)
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HY-P701588
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