LAIR1 蛋白, Mouse (HEK293, His)

The LAIR1 protein functions as an inhibitory receptor that negatively regulates NK cells, B cells, and T cells. LAIR1 is activated by tyrosine phosphorylation, recruits PTPN6 and PTPN11, and exerts downstream inhibitory effects. LAIR1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived LAIR1 protein, expressed by HEK293 , with C-6*His labeled tag.

LAIR1 蛋白作为抑制性受体发挥作用，对自然杀伤 (NK) 细胞、B 细胞和 T 细胞的溶细胞功能产生组成性负调节影响。通过酪氨酸磷酸化激活后，LAIR1 招募并激活磷酸酶 PTPN6 和 PTPN11，从而产生下游抑制作用。值得注意的是，它可以减少 B 细胞受体连接引起的细胞内钙的增加。除了与含 SH2 磷酸酶的作用外，LAIR1 还独立调节 CD4+ T 细胞中细胞因子的产生，下调 IL2 和 IFNG，同时诱导转化生长因子 β 的分泌。它进一步调节 B 细胞中 IgG 和 IgE 的产生，以及 IL8、IL10 和 TNF 的分泌。LAIR1 的影响延伸到抑制增殖、诱导骨髓性白血病细胞系凋亡、防止 NF-kappa-B p65 亚基/RELA 的核转位，以及抑制这些细胞中 I-kappa-B α/CHUK 的磷酸化。此外，它阻碍外周血前体细胞分化为树突状细胞。在相互作用方面，LAIR1 与酪氨酸蛋白磷酸酶 PTPN6 和 PTPN11 的 SH2 结构域组成型结合，并以高亲和力与细胞外基质胶原结合，这是一种功能上重要的相互作用。

Mouse

HEK293

C-6*His

Q8BG84-1 (Q22-Y141)

52855  [NCBI]

QEGSLPDITIFPNSSLMISQGTFVTVVCSYSDKHDLYNMVRLEKDGSTFMEKSTEPYKTEDEFEIGPVNETITGHYSCIYSKGITWSERSKTLELKVIKENVIQTPAPGPTSDTSWLKTY

21-35 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.