LAMP2/CD107b 蛋白, Human (HEK293, His)

LAMP2 is a key lysosomal glycoprotein that plays crucial roles in lysosomal biogenesis, pH regulation, and autophagy. It directly inhibits the TMEM175 proton channel, optimizing lysosomal acidity for efficient hydrolase activity. LAMP2/CD107b Protein, Human (HEK293, His) is the recombinant human-derived LAMP2/CD107b protein, expressed by HEK293 , with C-6*His labeled tag.

LAMP2 是一种溶酶体膜糖蛋白，在溶酶体生物发生、溶酶体 pH 调节和自噬中发挥着关键作用。它通过直接抑制质子通道 TMEM175 充当溶酶体腔 pH 值的重要调节剂，促进最佳溶酶体酸化以获得有效的水解酶活性。LAMP2 在分子伴侣介导的自噬中发挥重要作用，参与各种应激期间蛋白质的溶酶体降解，并作为长寿命蛋白质正常周转的一部分。该功能涉及结合 GAPDH、NLRP3 和 MLLT11 等靶蛋白，并指导它们进行溶酶体降解。在伴侣介导的自噬中，LAMP2 在 HSPA8/HSC70 等伴侣的下游发挥作用，这些伴侣识别并结合底物蛋白，介导它们招募至溶酶体，LAMP2 促进其结合。此外，LAMP2对于自噬过程中自噬体与溶酶体的融合至关重要，影响自噬体内容物的降解。此外，LAMP2是通过MHCII介导的途径有效呈递外源抗原所必需的，因为它调节伴侣介导的自噬并减少MHCII对内源抗原的呈递，突出了其在细胞过程中的多方面作用。

Human

HEK293

C-6*His

P13473-1 (L29-F375)

3920  [NCBI]

LELNLTDSENATCLYAKWQMNFTVRYETTNKTYKTVTISDHGTVTYNGSICGDDQNGPKIAVQFGPGFSWIANFTKAASTYSIDSVSFSYNTGDNTTFPDAEDKGILTVDELLAIRIPLNDLFRCNSLSTLEKNDVVQHYWDVLVQAFVQNGTVSTNEFLCDKDKTSTVAPTIHTTVPSPTTTPTPKEKPEAGTYSVNNGNDTCLLATMGLQLNITQDKVASVININPNTTHSTGSCRSHTALLRLNSSTIKYLDFVFAVKNENRFYLKEVNISMYLVNGSVFSIANNNLSYWDAPLGSSYMCNKEQTVSVSGAFQINTFDLRVQPFNVTQGKYSTAQECSLDDDTI

60-120 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.