Large envelope 蛋白, HBV-A (Cell-Free, P17101, His)

Large envelope proteins exist in two topological conformations: “external” (Le-HBsAg) and “internal” (Li-HBsAg). In its external state, it mediates viral attachment to cellular receptors, initiates infection, determines species specificity, and promotes viral particle internalization via caveolin-mediated endocytosis. Large envelope Protein, HBV-A (Cell-Free, P17101, His) is the recombinant Virus-derived Large envelope protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of Large envelope Protein, HBV-A (Cell-Free, P17101, His) is 399 a.a., with molecular weight of 45.1 kDa.

大包膜蛋白表现出两种不同的拓扑构象，称为“外部”或Le-HBsAg，和“内部”或Li-HBsAg。在其外部构象中，该蛋白质充当将病毒附着到细胞受体、启动感染以及确定物种特异性和肝向性的关键介质。这种相互作用主要通过小窝蛋白介导的内吞作用促进病毒粒子内化，同时还促进病毒粒子膜与内体膜之间的融合。在其内部构象中，该蛋白质在病毒颗粒形态发生中起着关键作用，并作为基质蛋白发挥作用，与核衣壳建立接触。同时，中间包膜蛋白对病毒体出芽有显着贡献，诱导不依赖核衣壳的过程。该出芽过程导致形成直径为 22 nm、缺乏核衣壳的亚病毒脂蛋白颗粒。

Virus

E. coli Cell-free

N-10*His

P17101 (G2-I400)

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GGWSSKPRKGMGTNLSVPNPLGFFPDHQLDPVFGANSNNPDWDFNPIKDHWPAANQVGVGAFGPGFTPPHGGVLGWSPQAQGMLTPVSTIPPPASANRQSGRQPTPISPPLRDSHPQAMQWNSTAFHQALQDPRVRGLYFPAGGSSSGTVNPAPNIASHISSISARTGDPVTNMENITSGFLGPLPVLQAGFFLLTRILTIPQSLDSWWTSLNFLGGSPVCLGQNSRSPTSNHSPTSCPPICPGYRWMCLRRFIIFLFILLLCLIFLLVLLDYQGMLPVCPLILGSTTTSTGPCKTCTTPAQGNSMFPSCCCTKPTDGNCTCIPIPSSWAFAKYLWEWASVRFSWLSLLVPFVQWFVGLSPTVWLSAIWMMWYWGPSLYSIVSSFIPLLPIFFCLWVYI

45.1 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.