1. 重组蛋白
  2. Viral Proteins
  3. Large envelope 蛋白, HBV-C (P.pastoris, 180a.a, His)

Large envelope 蛋白, HBV-C (P.pastoris, 180a.a, His)

目录号: HY-P702559
产品使用指南

大包膜蛋白的两种构象至关重要:“外部”(Le-HBsAg)和“内部”(Li-HBsAg)。在外部,它将病毒与细胞受体结合,启动感染,确定物种特异性,并通过小窝蛋白介导的内吞作用促进病毒颗粒内化。Large envelope 蛋白, HBV-C (P.pastoris, 180a.a, His) 是重组的 大包膜蛋白,由 P. pastoris 表达,带有 N-6*His 标签。Large envelope 蛋白, HBV-C (P.pastoris, 180a.a, His) 全长 180 个氨基酸,分子量为 20.9 kDa。

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描述

The large envelope protein is crucial in its two conformations, "outside" (Le-HBsAg) and "inside" (Li-HBsAg). Externally, it binds the virus to cellular receptors, initiates infection, determines species specificity, and promotes viral particle internalization via caveolin-mediated endocytosis. Large envelope Protein, HBV-C (P.pastoris, 180a.a, His) is the recombinant Virus-derived Large envelope protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of Large envelope Protein, HBV-C (P.pastoris, 180a.a, His) is 180 a.a., with molecular weight of 20.9 kDa.

研究背景

大包膜蛋白以两种拓扑构象存在,即“外部”或Le-HBsAg,和“内部”或Li-HBsAg。在其外部构象中,该蛋白质充当关键剂,将病毒与细胞受体结合并启动感染。这种关键的相互作用不仅建立了物种特异性和肝向性,而且还触发病毒颗粒内化,主要是通过小窝蛋白介导的内吞作用。大包膜蛋白进一步促进病毒体膜和内体膜之间的融合。在其内部构象中,该蛋白质在病毒颗粒形态发生中起着至关重要的作用,其功能类似于基质蛋白并介导与核衣壳的接触。同时,中间包膜蛋白在病毒体出芽中起着至关重要的作用,诱导不依赖核衣壳的过程。这种出芽机制导致亚病毒脂蛋白颗粒的形成,直径为 22 nm,不含核衣壳。

种属

Virus

表达系统

P. pastoris

标签

N-6*His

蛋白编号

P31868 (G2-G181)

基因 ID

/

蛋白结构
N-term
6*His
HBV-C L (G2-G181)
Accession # P31868
C-term
同用名
S; Large envelope protein; L glycoprotein; L-HBsAg; LHB; Large S protein; Large surface protein; Major surface antigen
分子量

20.9 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Large envelope Protein, HBV-C (P.pastoris, 180a.a, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Large envelope Protein, HBV-C (P.pastoris, 180a.a, His)
目录号:
HY-P702559
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