Large envelope 蛋白, HBV-D (Cell-Free, His)

The large envelope protein has two conformations: “external” (Le-HBsAg) and “internal” (Li-HBsAg). In its external state, it mediates viral attachment, initiates infection, determines species specificity, and promotes viral particle internalization via caveolin-mediated endocytosis. Large envelope Protein, HBV-D (Cell-Free, His) is the recombinant Virus-derived Large envelope protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of Large envelope Protein, HBV-D (Cell-Free, His) is 388 a.a., with molecular weight of Monomer:40 kDa Dimer:80 kDa.

大包膜蛋白表现出两种不同的拓扑构象，称为“外部”或 Le-HBsAg 和“内部”或 Li-HBsAg。在其外部构象中，该蛋白质充当关键介质，将病毒附着在细胞受体上并启动感染。这种相互作用决定了物种特异性和肝向性，主要通过小窝蛋白介导的内吞作用促进病毒颗粒内化。此外，大包膜蛋白促进病毒体膜和内体膜之间的融合。在其内部构象中，该蛋白质在病毒颗粒形态发生中起着至关重要的作用，其功能类似于基质蛋白并介导与核衣壳的接触。同时，中间包膜蛋白在病毒体出芽中发挥着至关重要的作用，诱导不依赖于核衣壳的过程。这一出芽过程导致大多数包膜蛋白形成直径为 22 nm 的亚病毒脂蛋白颗粒，不含核衣壳。

Virus

E. coli Cell-free

N-10*His

P03138 (G2-I389)

/

GQNLSTSNPLGFFPDHQLDPAFRANTANPDWDFNPNKDTWPDANKVGAGAFGLGFTPPHGGLLGWSPQAQGILQTLPANPPPASTNRQSGRQPTPLSPPLRNTHPQAMQWNSTTFHQTLQDPRVRGLYFPAGGSSSGTVNPVLTTASPLSSIFSRIGDPALNMENITSGFLGPLLVLQAGFFLLTRILTIPQSLDSWWTSLNFLGGTTVCLGQNSQSPTSNHSPTSCPPTCPGYRWMCLRRFIIFLFILLLCLIFLLVLLDYQGMLPVCPLIPGSSTTSTGPCRTCMTTAQGTSMYPSCCCTKPSDGNCTCIPIPSSWAFGKFLWEWASARFSWLSLLVPFVQWFVGLSPTVWLSVIWMMWYWGPSLYSILSPFLPLLPIFFCLWVYI

Monomer:40 kDa Dimer:80 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, 0.05% Brij-78, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add 5-50% of glycerol (final concentration). Our default final concentration of glycerol is 50%. Customers could use it as reference.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.