1. 重组蛋白
  2. Cytokines and Growth Factors Immune Checkpoint Proteins CD Antigens
  3. TNF Superfamily Stimulatory Immune Checkpoint Molecules Macrophage CD Proteins Monocyte CD Proteins
  4. TNF Superfamily Ligands LIGHT LIGHT/CD258
  5. LIGHT Proteins
  6. LIGHT/TNFSF14 蛋白, Mouse (sf9, His, solution)

LIGHT/TNFSF14 蛋白, Mouse (sf9, His, solution)

目录号: HY-P73837A
COA 产品使用指南

LIGHT蛋白 (CD258; TNFRSF14) 是由活化 T 细胞产生的 II 型跨膜蛋白,是 TNFRSF14/HVEM 配体,属于肿瘤坏死因子 (TNF) 家族。LIGHT 蛋白主要在脾脏中表达,具有膜结合型和可溶性型两种亚型。LIGHT蛋白可以作为肿瘤的免疫检查点分子,并刺激自然杀伤细胞产生干扰素 TFNγ,触发肿瘤凋亡信号。LIGHT 蛋白还参与 LTβR-NIK-p52 NF-κB 的显性通路促进炎症基因表达。此外,LIGHT 蛋白是激活淋巴样细胞的共刺激因子,对疱疹病毒感染有抑制作用。小鼠 LIGHT/TNFSF14 蛋白含有 239 个氨基酸和一个跨膜结构域 (38-58 a.a.)。重组小鼠 LIGHT/TNFSF14 蛋白 (sf9, His, solution) 是溶液形式的小鼠 LIGHT/TNFSF14 蛋白的胞外部分 (D72-V239),由 Sf9 昆虫细胞表达,具有 N 端 His 标记。

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描述

LIGHT/TNFSF14 protein (CD258; TNFRSF14) is a type II transmembrane protein produced by activated T cells. It is a TNFRSF14/HVEM ligand and belongs to the tumor necrosis factor (TNF) family. LIGHT/TNFSF14 is mainly expressed in spleen, with two subtypes: membrane-bound type and soluble type [1]. LIGHT/TNFSF14 protein can be used as immune checkpoint molecule of tumor, and stimulate natural killer cells to produce interferon TFNγ, triggering tumor apoptosis signal [2]. LIGHT/TNFSF14 is also involved in the dominant LTβR-NIK-p52 NF-κB pathway promoting the expression of inflammatory gene[3]. In addition, LIGHT/TNFSF14 protein is a co-stimulatory factor that activates lymphoid cells and has an inhibitory effect on herpes virus infection[4]. Mouse LIGHT/TNFSF14 Protein has 239 amino acids and a transmembrane domain (38-58 a.a.). LIGHT/TNFSF14 Protein, Mouse (sf9, His, solution) is the extracellular part (D72-V239) of mouse LIGHT/TNFSF14 Protein in solution form, produced by Sf9 insect cells, with N-terminal His-tag.

研究背景

LIGHT/TNFSF14是由活化的T细胞产生的II型跨膜蛋白,属于肿瘤坏死因子(TNF)家族。LIGHT/TNFSF14 是 TNFRSF14/HVEM(疱疹病毒进入介质)配体,与 LTalphabeta 异三聚体受体结合,但不与分泌性淋巴毒素 α (LTalpha) 或 LTbeta[1] 形成复合物。
LIGHT/TNFSF14 主要在脾脏中表达,但也在大脑中发现。在外周淋巴组织以及心脏、胎盘、肝脏、肺、阑尾和肾脏中微弱表达,在胎儿组织、内分泌腺或非造血肿瘤系中未见表达[1]
LIGHT/TNFSF14具有跨膜烷,因此可以分成2条链:膜形式和可溶形式。同工型 1 的可溶形式是通过蛋白水解加工从膜形式衍生而来。
在肿瘤免疫学中,TNFSF14/LIGHT 也可作为多形性胶质母细胞瘤 (GBM) 以及肺癌、乳腺癌、宫颈癌和前列腺癌。TNFSF14/LIGHT 可通过核因子-κB (NFκB) RelA/p50 信号传导刺激 NK 细胞产生 IFNγ。TNFSF14/LIGHT 维持 CD8+ 效应 T 细胞的功能,触发多种肿瘤细胞的凋亡[2]
在细胞信号传导中,TNFSF14/LIGHT 结合淋巴毒素-β 受体 (LTβR) 和 HVEM 可激活两者,并破坏表面结合形式的 HVEM-BTLA 复合物,并促进可溶形式的 HVEM-BTLA 复合物形成[2]
TNFSF14/LIGHT 通过 LTβR-NIK-p52 NF-κB 显性通路在体外促进炎症性食管成纤维细胞的形成,促进炎症基因表达并下调 WNT、BMP 和 3 型信号蛋白等稳态因子[3]
除此之外,TNFSF14/LIGHT 蛋白是淋巴细胞活化的共刺激因子,可阻止疱疹病毒感染。TNFSF14/LIGHT 还可预防肿瘤坏死因子 α 介导的原代肝细胞凋亡[4][5]

体外研究
(In Vitro)

LIGHT/TNFSF14 is homologous to lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for herpes virus entry mediator (HVEM), a receptor expressed by T lymphocytes[8].
LIGHT/TNFSF14 transgenic expression on T cells in mice promotes inflammation in multiple organs, including intestine[8].
LIGHT/TNFSF14-/- mice also shows increased accumulation of innate immune cells and higher levels of cytokines than colons from control mice, indicating a function for inflammation regulation in the colon[8].

体内研究
(In Vivo)

LIGHT/TNFSF14 (mouse; 20 μg/mL; 48 h) promotes insulin signalling in L6 skeletal muscle myotubes as indicated by increased expression of phospho-AKT[6].
LIGHT/TNFSF14 (mouse; 100 ng/mL; 24 h) reduces palmitate-induced insulin resistance and promotes insulin secretion in vitro[7].

种属

Mouse

表达系统

Sf9 insect cells

标签

N-His

蛋白编号

Q9QYH9 (D72-V239)

基因 ID

50930  [NCBI]

蛋白结构
N-term
His
LIGHT (D72-V239)
Accession # Q9QYH9
C-term
中文名
重组小鼠 LIGHT/TNFSF14 蛋白 (sf9, His, solution)
同用名
Tumor necrosis factor ligand superfamily member 14; TNFSF14; HVEM-L; LIGHT  
分子量

Approximately 20.5 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of 20 mM Tris, pH 8.0, 500 mM NaCl, 10 % gly.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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LIGHT/TNFSF14 Protein, Mouse (sf9, His, solution)
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HY-P73837A
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