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  3. Major urinary 蛋白 11 蛋白, Mouse (His)

Major urinary 蛋白 11 蛋白, Mouse (His)

目录号: HY-P71506
产品使用指南

主要尿蛋白 11 蛋白在信息素结合、稳定和促进其从尿痕中缓慢释放方面发挥着关键作用。它可以保护信息素免遭氧化,并有可能充当信息素本身,影响攻击、交配、哺乳、领地建立和统治等社会行为。该蛋白在体外表现出与信息素类似物 2-仲丁基-4,5-二氢噻唑 (SBT) 的结合。Major urinary 蛋白 11 蛋白, Mouse (His) 是重组的 主要尿蛋白 11 蛋白,由 E. coli 表达,带有 N-His 标签。Major urinary 蛋白 11 蛋白, Mouse (His) 全长 151 个氨基酸,分子量约为 ~23.6 kDa。

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描述

Major urinary protein 11 Protein functions as a key player in pheromone binding, stabilizing and facilitating their slow release from urine marks. It may safeguard pheromones from oxidation and potentially acts as a pheromone itself, influencing social behaviors like aggression, mating, pup-suckling, territory establishment, and dominance. The protein exhibits in vitro binding to the pheromone analog 2-sec-butyl-4,5-dihydrothiazole (SBT). Major urinary protein 11 Protein, Mouse (His) is the recombinant mouse-derived Major urinary protein 11 protein, expressed by E. coli , with N-His labeled tag. The total length of Major urinary protein 11 Protein, Mouse (His) is 151 a.a., with molecular weight of ~23.6 kDa.

研究背景

主要尿蛋白 11 蛋白是主要尿蛋白 (Mups) 家族的一员,在结合信息素、稳定信息素以通过尿痕缓慢释放到空气中方面发挥着至关重要的作用。这种功能可以保护信息素免受氧化,有趣的是,Mups 本身也可以充当信息素。在包括攻击、交配、哺乳、领地建立和支配等社会行为的背景下,主要尿蛋白 11 被认为有助于这些行为的调节。此外,体外研究表明它能够结合信息素类似物 2-仲丁基-4,5-二氢噻唑 (SBT)。

种属

Mouse

表达系统

E. coli

标签

N-His

蛋白编号

P04938 (1R-151E)

基因 ID

100039028  [NCBI]

蛋白结构
N-term
His
Mup11 (1R-151E)
Accession # P04938
C-term
同用名
Mup11; Mup9Major urinary protein 11
氨基酸序列

REKINGEWHTIILASDKREKIEDNGNFRLFLEQIHVLENSLVLKFHTVRDEECSELSMVADKTEKAGEYSVTYDGFNTFTIPKTDYDNFLMAHLINEKDGETFQLMGLYGREPDLSSDIKERFAQLCEEHGILRENIIDLSNANRCLQARE

分子量

Approximately 23.6 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Major urinary protein 11 Protein, Mouse (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Major urinary protein 11 Protein, Mouse (His)
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