2. 重组蛋白
  3. Enzymes & Regulators
  4. Transferases (EC 2)
  5. MAPK4 蛋白, Human (Sf9, His, GST)

MAPK4 蛋白, Human (Sf9, His, GST)

目录号: HY-P701803
产品使用指南

MAPK4 是一种非典型 MAPK 蛋白，可磷酸化微管相关蛋白 2 (MAP2) 和 MAPKAPK5，形成具有复杂磷酸化事件的复合物。与 MAPKAPK5 的相互作用导致 ERK3/MAPK6 在 Ser-189 处磷酸化，从而激活 MAPKAPK5，从而相互磷酸化 ERK3/MAPK6。这种相互作用的具体细胞作用虽然尚不清楚，但表明 ERK3 可能参与促进进入细胞周期。MAPK4 蛋白, Human (Sf9, His, GST) 是重组的 MAPK4 蛋白，由 Sf9 insect cells 表达，带有 N-8*His, N-GST 标签。MAPK4 蛋白, Human (Sf9, His, GST) 全长 586 个氨基酸。

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描述

MAPK4, an atypical MAPK protein, phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5, forming a complex with intricate phosphorylation events. Interaction with MAPKAPK5 leads to ERK3/MAPK6 phosphorylation at Ser-189, activating MAPKAPK5, which reciprocally phosphorylates ERK3/MAPK6. The specific cellular role of this interplay, while unclear, suggests ERK3's potential involvement in promoting entry into the cell cycle. MAPK4 Protein, Human (Sf9, His, GST) is the recombinant human-derived MAPK4 protein, expressed by Sf9 insect cells , with N-8*His, N-GST labeled tag. The total length of MAPK4 Protein, Human (Sf9, His, GST) is 586 a.a., .
研究背景

ERK3 是一种非典型 MAPK 蛋白，通过磷酸化微管相关蛋白 2 (MAP2) 和 MAPKAPK5 表现出独特的激酶活性。MAPKAPK5 形成的复合物的功能意义尚未完全阐明，但它涉及一系列复杂的磷酸化事件。在与非典型 MAPKAPK5 相互作用后，ERK3/MAPK6 在 Ser-189 处发生磷酸化，随后促进 MAPKAPK5 的磷酸化和激活。有趣的是，MAPKAPK5 会相互磷酸化 ERK3/MAPK6。这种相互作用在细胞过程中的具体作用仍不清楚，尽管有人认为 ERK3 可能在促进进入细胞周期方面发挥作用。
种属

Human
表达系统

Sf9 insect cells
标签

N-8*His;N-GST
蛋白编号

P31152 (A2-W587)
基因 ID

5596
蛋白结构
N-term
8*His-GST
MAPK4 (A2-W587)
Accession # P31152
C-term
同用名
MAPK4; Mitogen-activated protein kinase 4; MAP kinase 4; MAPK 4; Extracellular signal-regulated kinase 4; ERK-4; MAP kinase isoform p63; p63-MAPK
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

MAPK4 Protein, Human (Sf9, His, GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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