1. 重组蛋白
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. MDH2 蛋白, Human (HEK293, His)

MDH2 蛋白, Human (HEK293, His)

目录号: HY-P70350
COA 产品使用指南

MDH2 是一种经历乙酰化的关键酶,导致其酶活性增加。这种翻译后修饰充当 MDH2 的调节机制,可能影响其功能并在细胞代谢过程的调节中发挥作用。MDH2 蛋白, Human (HEK293, His) 是重组的 MDH2 蛋白,由 HEK293 表达,带有 C-6*His 标签。MDH2 蛋白, Human (HEK293, His) 全长 314 个氨基酸,分子量约为 ~34.0 kDa。

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规格 价格 是否有货 数量
10 μg ¥1600 In-stock
50 μg ¥4500 In-stock
100 μg   询价  

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  • 生物活性

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描述

MDH2 is a key enzyme that undergoes acetylation, leading to an increase in its enzymatic activity. This post-translational modification acts as a regulatory mechanism for MDH2, potentially affecting its function and playing a role in the regulation of cellular metabolic processes. MDH2 Protein, Human (HEK293, His) is the recombinant human-derived MDH2 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of MDH2 Protein, Human (HEK293, His) is 314 a.a., with molecular weight of ~34.0 kDa.

研究背景

MDH2 是一种关键酶,会发生乙酰化,从而导致其酶活性增加。这种翻译后修饰充当 MDH2 的调节机制,可能影响其功能并在细胞代谢过程的调节中发挥作用。了解乙酰化对 MDH2 的具体影响可以深入了解代谢途径的调节,并可能对与代谢失调相关的疾病产生影响。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

P40926 (A25-K338)

基因 ID
蛋白结构
N-term
MDH2 (A25-K338)
Accession # P40926
6*His
C-term
同用名
rHuMalate dehydrogenase mitochondrial/MDH2, His ; Malate dehydrogenase; mitochondrial; MDH2
氨基酸序列

AKVAVLGASGGIGQPLSLLLKNSPLVSRLTLYDIAHTPGVAADLSHIETKAAVKGYLGPEQLPDCLKGCDVVVIPAGVPRKPGMTRDDLFNTNATIVATLTAACAQHCPEAMICVIANPVNSTIPITAEVFKKHGVYNPNKIFGVTTLDIVRANTFVAELKGLDPARVNVPVIGGHAGKTIIPLISQCTPKVDFPQDQLTALTGRIQEAGTEVVKAKAGAGSATLSMAYAGARFVFSLVDAMNGKEGVVECSFVKSQETECTYFSTPLLLGKKGIEKNLGIGKVSSFEEKMISDAIPELKASIKKGEDFVKTLK

分子量

Approximately 34.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

MDH2 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
MDH2 Protein, Human (HEK293, His)
目录号:
HY-P70350
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