MEK2 蛋白, Human (sf9, GST)

MEK2, a pivotal kinase in cellular signaling, catalyzes the simultaneous phosphorylation of a threonine and a tyrosine residue within the Thr-Glu-Tyr sequence of MAP kinases. This enzymatic activity results in the activation of ERK1 and ERK2 MAP kinases, contributing to the intricate network of cellular responses. MEK2's role extends to the activation of BRAF, a process regulated by its interaction with KSR1 or KSR2. Binding to KSR1 or KSR2 facilitates the release of inhibitory intramolecular interactions within KSR1 or KSR2, promoting the dimerization of KSR1 or KSR2 with BRAF and subsequent BRAF activation. This mechanism highlights MEK2's significance in orchestrating signaling cascades critical for cellular functions. MEK2 Protein, Human (sf9, GST) is the recombinant human-derived MEK2 protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of MEK2 Protein, Human (sf9, GST) is 400 a.a., with molecular weight of ~66 kDa.

MEK2 是细胞信号转导中的关键激酶，催化 MAP 激酶的 Thr-Glu-Tyr 序列中的苏氨酸和酪氨酸残基同时磷酸化。这种酶活性会激活 ERK1 和 ERK2 MAP 激酶，从而形成复杂的细胞反应网络。 MEK2 的作用延伸至 BRAF 的激活，这是一个由其与 KSR1 或 KSR2 相互作用调节的过程。与 KSR1 或 KSR2 的结合促进 KSR1 或 KSR2 内抑制性分子内相互作用的释放，促进 KSR1 或 KSR2 与 BRAF 的二聚化以及随后的 BRAF 激活。这种机制凸显了 MEK2 在协调对细胞功能至关重要的信号级联中的重要性。

Human

Sf9 insect cells

N-GST

P36507 (M1-V400)

5605  [NCBI]

Approximately 66 kDa

Greater than 90% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.