1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. MGST2 蛋白, Human (Cell-Free, His)

MGST2 蛋白, Human (Cell-Free, His)

目录号: HY-P702379
产品使用指南

作为一种多功能酶,MGST2蛋白催化NAD(+)合成环状ADP-β-D-核糖(cADPR),随后将cADPR水解为ADP-D-核糖(ADPR)。这种双重功能使 MGST2 成为调节细胞内钙动员的关键角色,因为 cADPR 充当内源性第二信使,触发细胞内储存的钙的释放。MGST2 蛋白, Human (Cell-Free, His) 是重组的 MGST2 蛋白,由 E. coli Cell-free 表达,带有 N-10*His 标签。MGST2 蛋白, Human (Cell-Free, His) 全长 147 个氨基酸,分子量为 19.4 kDa。

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描述

As a multifunctional enzyme, MGST2 protein catalyzes the synthesis of cyclic ADP-β-D-ribose (cADPR) from NAD(+) and subsequently hydrolyzes cADPR into ADP-D-ribose (ADPR). This dual function makes MGST2 a key player in regulating intracellular calcium mobilization, as cADPR acts as an endogenous second messenger that triggers the release of intracellular stored calcium. MGST2 Protein, Human (Cell-Free, His) is the recombinant human-derived MGST2 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of MGST2 Protein, Human (Cell-Free, His) is 147 a.a., with molecular weight of 19.4 kDa.

研究背景

CD157 蛋白是一种具有双重功能的多功能酶,可催化 NAD(+) 合成环状 ADP-β-D-核糖 (cADPR) 以及随后将 cADPR 水解为 ADP-D-核糖 (ADPR)。合成的 cADPR 作为内源性第二信使,已知会触发细胞内储存的钙释放,从而在调节细胞内钙动员中发挥关键作用(可能)。除了其酶活性外,CD157 还与前 B 细胞生长有关,表明在与 B 细胞成熟相关的发育过程中具有潜在作用(可能)。

种属

Human

表达系统

E. coli Cell-free

标签

N-10*His

蛋白编号

Q99735 (M1-F147)

基因 ID

4258

蛋白结构
N-term
10*His
MGST2 (M1-F147)
Accession # Q99735
C-term
同用名
Microsomal glutathione S-transferase 2; Glutathione peroxidase MGST2; 1.11.1.-; Leukotriene C4 synthase MGST2; 4.4.1.20; Microsomal glutathione S-transferase II; Microsomal GST-II
氨基酸序列

MAGNSILLAAVSILSACQQSYFALQVGKARLKYKVTPPAVTGSPEFERVFRAQQNCVEFYPIFIITLWMAGWYFNQVFATCLGLVYIYGRHLYFWGYSEAAKKRITGFRLSLGILALLTLLGALGIANSFLDEYLDLNIAKKLRRQF

分子量

19.4 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

MGST2 Protein, Human (Cell-Free, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
MGST2 Protein, Human (Cell-Free, His)
目录号:
HY-P702379
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