NFKB1 蛋白, Human (His)

The NFKB1 protein is a subunit of NF-kappa-B and regulates transcription in a variety of biological processes. It forms homodimeric or heterodimeric complexes with RELA/p65, RELB, and NFKB2/p52. NFKB1 Protein, Human (His) is the recombinant human-derived NFKB1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of NFKB1 Protein, Human (His) is 434 a.a., with molecular weight of 45-55 kDa.

NFKB1 蛋白是多效性转录因子 NF-kappa-B 的关键成分，广泛存在于各种细胞类型中。该转录因子在与多种生物过程相关的信号转导途径中充当中心介质，包括炎症、免疫、分化、细胞生长、肿瘤发生和细胞凋亡。NFKB1 参与同源或异源二聚体复合物，其中 p65-p50 异源二聚体最为普遍。这些二聚体选择性地结合靶基因 DNA 中的 kappa-B 位点，表现出不同的偏好和亲和力。NF-κ-B 调节涉及复杂的翻译后修饰、亚细胞区室化以及与辅因子或辅阻遏物的相互作用。在非活性状态下，NF-kappa-B 复合物与细胞质中的 NF-kappa-B 抑制剂 (I-kappa-B) 家族成员结合。通过 I-kappa-B 的磷酸化和降解而激活，释放活性 NF-kappa-B 复合物以进行核易位。NFKB1 具有双重功能，有助于 NF-kappa-B 蛋白在细胞质中的保留，并通过共翻译加工产生活性 p50 亚基。这种由蛋白酶体介导的加工过程确保了 p50 和 p105 的独立功能。p50 亚基与参与免疫反应和急性期反应的基因增强子区域中的 kappa-B 共有序列结合。此外，NFKB1 与 MAP3K8 结合，抑制 MAP3K8 诱导的 MAPK 信号传导，通过 NFKB1/p105 的蛋白酶体依赖性降解释放活性 MAP3K8。

Human

E. coli

N-6*His

P19838-2 (M1-G434)

4790  [NCBI]

MAEDDPYLGRPEQMFHLDPSLTHTIFNPEVFQPQMALPTADGPYLQILEQPKQRGFRFRYVCEGPSHGGLPGASSEKNKKSYPQVKICNYVGPAKVIVQLVTNGKNIHLHAHSLVGKHCEDGICTVTAGPKDMVVGFANLGILHVTKKKVFETLEARMTEACIRGYNPGLLVHPDLAYLQAEGGGDRQLGDREKELIRQAALQQTKEMDLSVVRLMFTAFLPDSTGSFTRRLEPVVSDAIYDSKAPNASNLKIVRMDRTAGCVTGGEEIYLLCDKVQKDDIQIRFYEEEENGGVWEGFGDFSPTDVHRQFAIVFKTPKYKDINITKPASVFVQLRRKSDLETSEPKPFLYYPEIKDKEEVQRKRQKLMPNFSDSFGGGSGAGAGGGGMFGSGGGGGGTGSTGPGYSFPHYGFPTYGGITFHPGTTKSNAGMKHG

45-55 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 20 mM GSH, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.