Nucleolar transcription factor 1/UBTF 蛋白, Human (His-SUMO)

The nucleolar transcription factor 1/UBTF protein critically activates RNA polymerase I-mediated transcription by binding to the ribosomal RNA gene promoter and cooperating with the SL1/TIF-IB complex. Nucleolar transcription factor 1/UBTF Protein, Human (His-SUMO) is the recombinant human-derived Nucleolar transcription factor 1/UBTF protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag. The total length of Nucleolar transcription factor 1/UBTF Protein, Human (His-SUMO) is 670 a.a., with molecular weight of ~95.0 kDa.

核仁转录因子 1/UBTF 蛋白通过识别核糖体 RNA 基因启动子并与转录因子 SL1/TIF-IB 复合物协同相互作用，在 RNA 聚合酶 I 介导的转录激活中发挥着至关重要的作用。UBTF 与上游控制元件特异性结合，作为同二聚体发挥作用，并与各种蛋白质相互作用，包括 TBP、TAF1A、RASL11A、IRS1、PIK3CA、DHX33、PHF6、CEBPA（异构体 1 和异构体 4）、DDX11、NOP53 和 ALKBH2。这些相互作用突出了该蛋白质参与多种细胞过程，强调其在核糖体 RNA 基因转录中的调节作用及其与转录控制和核仁功能中涉及的关键因素的关联。

Human

E. coli

N-SUMO;N-6*His

P17480 (M1-T670)

7343  [NCBI]

MNGEADCPTDLEMAAPKGQDRWSQEDMLTLLECMKNNLPSNDSSKFKTTESHMDWEKVAFKDFSGDMCKLKWVEISNEVRKFRTLTELILDAQEHVKNPYKGKKLKKHPDFPKKPLTPYFRFFMEKRAKYAKLHPEMSNLDLTKILSKKYKELPEKKKMKYIQDFQREKQEFERNLARFREDHPDLIQNAKKSDIPEKPKTPQQLWYTHEKKVYLKVRPDATTKEVKDSLGKQWSQLSDKKRLKWIHKALEQRKEYEEIMRDYIQKHPELNISEEGITKSTLTKAERQLKDKFDGRPTKPPPNSYSLYCAELMANMKDVPSTERMVLCSQQWKLLSQKEKDAYHKKCDQKKKDYEVELLRFLESLPEEEQQRVLGEEKMLNINKKQATSPASKKPAQEGGKGGSEKPKRPVSAMFIFSEEKRRQLQEERPELSESELTRLLARMWNDLSEKKKAKYKAREAALKAQSERKPGGEREERGKLPESPKRAEEIWQQSVIGDYLARFKNDRVKALKAMEMTWNNMEKKEKLMWIKKAAEDQKRYERELSEMRAPPAATNSSKKMKFQGEPKKPPMNGYQKFSQELLSNGELNHLPLKERMVEIGSRWQRISQSQKEHYKKLAEEQQKQYKVHLDLWVKSLSPQDRAAYKEYISNKRKSMTKLRGPNPKSSRTT

Approximately 95.0 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1.0 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.